Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
Associated disease: infection due to Bacillus subtilis [ICD11:
XM4SG9 
]
The structure was elucidated in this paperNCBI PubMed ID: 21514573Publication DOI: 10.1016/j.carres.2011.03.034Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: G.M. Streshinskaya <streshinskaya
mail.ru>
Institutions: School of Biology, M. V. Lomonosov Moscow State University, Moscow, Russian Federation
The cell wall of Bacillus subtilis VKM B-762 contains, along with 1,5-poly[4-O-(2-acetamido-2-deoxy-β-d-glucopyranosyl)ribitol phosphate], a novel type of glycopolymer involving three types of inter-monomeric bonds in the repeating unit, viz., amide, glycosidic and phosphodiester: [See figure in text]. Such a structural pattern of natural glycopolymers has been hitherto unknown. This polymer represents a novel type of teichoic acids.
cell wall, teichoic acid, Bacillus, Bacillus subtilis, NMR-spectroscopy
Structure type: polymer chemical repeating unit
Location inside paper: abstract, p. 1175, table 1, polymer B
Trivial name: WTA, cell wall teichoic acid
Compound class: cell wall polysaccharide
Methods: 13C NMR, 1H NMR, deacetylation, NMR-2D, partial acid hydrolysis, HF solvolysis, 31P NMR, ESI-MS, composition analysis, peracetylation
Related record ID(s): 26345, 26455, 26456, 26458, 26459
NCBI Taxonomy refs (TaxIDs): 1423
Show glycosyltransferases
NMR conditions: in D2O at 303 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
0,1,4 l?GroA 178.1 78.2 63.9
0,1 bDQuip4N 104.1 75.1 74.6 58.3 72.0 18.2
0 x?Gro 72.3 70.6 68.0
P
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
0,1,4 l?GroA - 4.66 3.96
0,1 bDQuip4N 4.50 3.40 3.69 3.69 3.69 1.22
0 x?Gro 3.73-4.01 4.07 3.96-4.05
P
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
0,1,4 l?GroA 78.2/4.66 63.9/3.96
0,1 bDQuip4N 104.1/4.50 75.1/3.40 74.6/3.69 58.3/3.69 72.0/3.69 18.2/1.22
0 x?Gro 72.3/3.73-4.01 70.6/4.07 68.0/3.96-4.05
P
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 0,1,4 | l?GroA |
| 4.66 | 3.96 | |
| 0,1 | bDQuip4N | 4.50 | 3.40 | 3.69 | 3.69 | 3.69 | 1.22 |
| 0 | x?Gro | 3.73
4.01 | 4.07 | 3.96
4.05 | |
| | P | |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 0,1,4 | l?GroA | 178.1 | 78.2 | 63.9 | |
| 0,1 | bDQuip4N | 104.1 | 75.1 | 74.6 | 58.3 | 72.0 | 18.2 |
| 0 | x?Gro | 72.3 | 70.6 | 68.0 | |
| | P | |
|
There is only one chemically distinct structure:
Found 
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