Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
Associated disease: infection due to Bacillus subtilis [ICD11:
XM4SG9 
]
The structure was elucidated in this paperNCBI PubMed ID: 21514573Publication DOI: 10.1016/j.carres.2011.03.034Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: G.M. Streshinskaya <streshinskaya
mail.ru>
Institutions: School of Biology, M. V. Lomonosov Moscow State University, Moscow, Russian Federation
The cell wall of Bacillus subtilis VKM B-762 contains, along with 1,5-poly[4-O-(2-acetamido-2-deoxy-β-d-glucopyranosyl)ribitol phosphate], a novel type of glycopolymer involving three types of inter-monomeric bonds in the repeating unit, viz., amide, glycosidic and phosphodiester: [See figure in text]. Such a structural pattern of natural glycopolymers has been hitherto unknown. This polymer represents a novel type of teichoic acids.
cell wall, teichoic acid, Bacillus, Bacillus subtilis, NMR-spectroscopy
Structure type: oligomer
Location inside paper: p. 1175, table 1, glycoside B
Methods: 13C NMR, 1H NMR, deacetylation, NMR-2D, partial acid hydrolysis, HF solvolysis, 31P NMR, ESI-MS, composition analysis, peracetylation
Related record ID(s): 26345, 26455, 26456, 26457, 26459
NCBI Taxonomy refs (TaxIDs): 1423
Show glycosyltransferases
NMR conditions: in D2O at 303 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
1,4 l?GroA 176.3 73.7 64.5
1 bDQuip4N 103.8 75.0 74.4 57.7 72.2 18.0
x?Gro 72.2 71.9 63.6
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
1,4 l?GroA - 4.27 3.80-3.83
1 bDQuip4N 4.45 3.37 3.62 3.64 3.64 1.19
x?Gro 3.64-3.95 3.92 3.58-3.65
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
1,4 l?GroA 73.7/4.27 64.5/3.80-3.83
1 bDQuip4N 103.8/4.45 75.0/3.37 74.4/3.62 57.7/3.64 72.2/3.64 18.0/1.19
x?Gro 72.2/3.64-3.95 71.9/3.92 63.6/3.58-3.65
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 1,4 | l?GroA |
| 4.27 | 3.80
3.83 | |
| 1 | bDQuip4N | 4.45 | 3.37 | 3.62 | 3.64 | 3.64 | 1.19 |
| | x?Gro | 3.64
3.95 | 3.92 | 3.58
3.65 | |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 1,4 | l?GroA | 176.3 | 73.7 | 64.5 | |
| 1 | bDQuip4N | 103.8 | 75.0 | 74.4 | 57.7 | 72.2 | 18.0 |
| | x?Gro | 72.2 | 71.9 | 63.6 | |
|
There is only one chemically distinct structure:
Found 
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