Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Sus scrofa domestica
Associated disease: septicemia [ICD11:
MA15.Y 
];
meningitis [ICD11:
1D01 ];
inflammatory arthropathies [ICD11:
FA2Z ]
The structure was elucidated in this paperNCBI PubMed ID: 21612441Publication DOI: 10.1139/o11-001Journal NLM ID: 8606068Publisher: Ottawa: National Research Council of Canada
Correspondence: monteiro
uoguelph.ca
Institutions: Department of Chemistry, University of Guelph, Canada
We are developing a serotyping system for Actinobacillus suis based on its capsule (K) and lipopolysaccharide O-chain (O) structures. Previously, we have shown that less virulent strains of this swine pathogen express a (1→6)-β-D-glucan as both K- and O-chain polysaccharides and were serologically classified as K:1/O:1. Here, we show that representative A. suis strains with a high (H91-0380; serotype K:2/O:2) and intermediate (C84; serotype K:2/O:1) degree of virulence possess a capsule polysaccharide (K:2) composed of an O-acetylated diglycosyl phosphate repeat decorated with fructose: [→4)-3-O-Ac-β-D-GlcpNAc-(1→3)-[β-D-Fruf-(2→2)]-α-D-Galp-(1→PO(4)(-)→]. In addition, the serotype O:2 lipopolysaccharide was shown to express a sialylated O-chain [→3)-β-D-Galp-(1→4)-[Neu5Ac-(2→3)-α-D-Galp-(1→6)]-β-D-Glcp-(1→6)-β-D-GlcpNAc-(1→]. As (1→6)-β-D-glucan is ubiquitous in the environment, low levels of antibodies in the animals are predicted to prevent disease by K:1/O:1 strains. The greater potential associated with K:2/O:2 and K:2/O:1 strains is most likely due to the absence of (1→6)-β-D-glucan as the K antigen and, in the case of K:2/O:2, the presence of sialic acid in the lipopolysaccharide, a nonulosonic acid known to promote evasion of host recognition.
Lipopolysaccharide, sialic acid, fructose, capsule polysaccharide, Actinobacillus suis
Structure type: polymer chemical repeating unit
Location inside paper: abstract, p.328
Compound class: CPS
Contained glycoepitopes: IEDB_135813,IEDB_136906,IEDB_137340,IEDB_137472,IEDB_141794,IEDB_141807,IEDB_145001,IEDB_151528,IEDB_151531,IEDB_190606,SB_173,SB_7
Methods: 13C NMR, 1H NMR, NMR-2D, GC-MS, sugar analysis, 31P NMR, de-O-acetylation, NMR-1D, defructosylation, computational methods
Comments, role: de-O-acetylated CPS from Actinobacillus suis C84 serotype K:2/O:1 and Actinobacillus suis H91-0380 serotype K:2/O:2
Related record ID(s): 26181, 26604, 26605
NCBI Taxonomy refs (TaxIDs): 716,
696748Reference(s) to other database(s): GTC:G87238KE
Show glycosyltransferases
NMR conditions: in D2O at 300 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
0,3,2 Ac
0,3 bDGlcpN 104.3 58.5 76.5 77.0 77.9 63.2
0,2 70%bDFruf 63.3 106.2 78.7 77.0 83.6 65.7
0 aDGalp 98.8 70.5 79.2 72.3 74.1 63.7
P
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
0,3,2 Ac
0,3 bDGlcpN 4.72 3.85 3.72 4.07 3.55 3.85-3.91
0,2 70%bDFruf 3.42-3.64 - 4.13 4.09 3.81 3.83-3.83
0 aDGalp 5.65 4.21 4.05 4.32 4.23 3.72
P
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
0,3,2 Ac
0,3 bDGlcpN 104.3/4.72 58.5/3.85 76.5/3.72 77.0/4.07 77.9/3.55 63.2/3.85-3.91
0,2 70%bDFruf 63.3/3.42-3.64 78.7/4.13 77.0/4.09 83.6/3.81 65.7/3.83-3.83
0 aDGalp 98.8/5.65 70.5/4.21 79.2/4.05 72.3/4.32 74.1/4.23 63.7/3.72
P
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 0,3,2 | Ac | |
| 0,3 | bDGlcpN | 4.72 | 3.85 | 3.72 | 4.07 | 3.55 | 3.85
3.91 |
| 0,2 | 70%bDFruf | 3.42
3.64 |
| 4.13 | 4.09 | 3.81 | 3.83
3.83 |
| 0 | aDGalp | 5.65 | 4.21 | 4.05 | 4.32 | 4.23 | 3.72 |
| | P | |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 0,3,2 | Ac | |
| 0,3 | bDGlcpN | 104.3 | 58.5 | 76.5 | 77.0 | 77.9 | 63.2 |
| 0,2 | 70%bDFruf | 63.3 | 106.2 | 78.7 | 77.0 | 83.6 | 65.7 |
| 0 | aDGalp | 98.8 | 70.5 | 79.2 | 72.3 | 74.1 | 63.7 |
| | P | |
|
There is only one chemically distinct structure:
Found 
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