Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Sus scrofa domestica
Associated disease: septicemia [ICD11:
MA15.Y 
];
meningitis [ICD11:
1D01 ];
inflammatory arthropathies [ICD11:
FA2Z ]
The structure was elucidated in this paperNCBI PubMed ID: 21612441Publication DOI: 10.1139/o11-001Journal NLM ID: 8606068Publisher: Ottawa: National Research Council of Canada
Correspondence: monteiro
uoguelph.ca
Institutions: Department of Chemistry, University of Guelph, Canada
We are developing a serotyping system for Actinobacillus suis based on its capsule (K) and lipopolysaccharide O-chain (O) structures. Previously, we have shown that less virulent strains of this swine pathogen express a (1→6)-β-D-glucan as both K- and O-chain polysaccharides and were serologically classified as K:1/O:1. Here, we show that representative A. suis strains with a high (H91-0380; serotype K:2/O:2) and intermediate (C84; serotype K:2/O:1) degree of virulence possess a capsule polysaccharide (K:2) composed of an O-acetylated diglycosyl phosphate repeat decorated with fructose: [→4)-3-O-Ac-β-D-GlcpNAc-(1→3)-[β-D-Fruf-(2→2)]-α-D-Galp-(1→PO(4)(-)→]. In addition, the serotype O:2 lipopolysaccharide was shown to express a sialylated O-chain [→3)-β-D-Galp-(1→4)-[Neu5Ac-(2→3)-α-D-Galp-(1→6)]-β-D-Glcp-(1→6)-β-D-GlcpNAc-(1→]. As (1→6)-β-D-glucan is ubiquitous in the environment, low levels of antibodies in the animals are predicted to prevent disease by K:1/O:1 strains. The greater potential associated with K:2/O:2 and K:2/O:1 strains is most likely due to the absence of (1→6)-β-D-glucan as the K antigen and, in the case of K:2/O:2, the presence of sialic acid in the lipopolysaccharide, a nonulosonic acid known to promote evasion of host recognition.
Lipopolysaccharide, sialic acid, fructose, capsule polysaccharide, Actinobacillus suis
Structure type: polymer chemical repeating unit
Location inside paper: abstract, p.330
Compound class: O-polysaccharide
Contained glycoepitopes: IEDB_135813,IEDB_136044,IEDB_136794,IEDB_136906,IEDB_137340,IEDB_137472,IEDB_1391966,IEDB_140529,IEDB_141794,IEDB_141807,IEDB_142351,IEDB_142487,IEDB_142488,IEDB_146100,IEDB_146664,IEDB_149138,IEDB_149139,IEDB_149141,IEDB_149174,IEDB_150933,IEDB_151528,IEDB_151531,IEDB_167069,IEDB_190606,IEDB_423085,IEDB_983931,SB_145,SB_165,SB_166,SB_170,SB_171,SB_172,SB_173,SB_187,SB_192,SB_195,SB_39,SB_6,SB_7,SB_84,SB_88
Methods: 13C NMR, 1H NMR, NMR-2D, GC-MS, sugar analysis, 31P NMR, de-O-acetylation, NMR-1D, defructosylation, computational methods
Comments, role: Actinobacillus suis C84 serotype K:2/O:1 and Actinobacillus suis H91-0380 serotype K:2/O:2.
Related record ID(s): 26181, 26603, 26605
NCBI Taxonomy refs (TaxIDs): 716,
696748Reference(s) to other database(s): GTC:G05143GV
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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