Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: pneumonia [ICD11:
CA40 
];
bacteremia [ICD11:
MA15.0 ];
meningitis [ICD11:
1D01 ];
urinary tract infections (UTI) [ICD11:
GC08 ];
infection due to Acinetobacter baumannii [ICD11:
XN8LS ]
NCBI PubMed ID: 21463855Publication DOI: 10.1016/j.carres.2011.03.024Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: decastro
unina.it
Institutions: Division of Structural Biochemistry, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Borstel, Germany, Università di Napoli Federico II, Dipartimento di Chimica Organica e Biochimica, Complesso Universitario Monte Sant’Angelo, Napoli, Italy
The structures of the capsular polysaccharides (CPSs) of the two clinical isolates Acinetobacter baumannii SMAL and MG1 were elucidated. Hot phenol/water extractions of the dry biomasses, followed by enzymatic digestions and repeated ultracentrifugations led to the isolation of polysaccharides that were negative in Western blot analysis utilizing an anti-lipid A antibody, thus proving that they were not the LPS O-antigens but CPSs. Their structures were established on the basis of NMR spectroscopy and GC-MS analyses. The A. baumannii MG1 CPS consisted of a linear aminopolysaccharide with acyl substitution heterogeneity at the N-4 amino group of QuipN4N: 4)-α-D-GlcpNAc-(1→4)-α-L-GalpNAcA-(1→3)-β-D-QuipNAc4NR-(1→ R=3-hydroxybutyrryl or acetyl. The repeating unit of the CPS produced by strain SMAL is a pentasaccharide, already reported for the O-antigen moiety from A. baumannii strain ATCC 17961: (formula, see text).
Acinetobacter baumannii, capsular polysaccharide, structural analysis, Western blot
Structure type: polymer chemical repeating unit
Location inside paper: abstract, p. 976, fig.6b
Compound class: CPS
Contained glycoepitopes: IEDB_137340,IEDB_141807,IEDB_151531
Methods: 13C NMR, 1H NMR, SDS-PAGE, sugar analysis, deacylation, Western blotting, NMR-1D
Related record ID(s): 26202, 26627, 26628
NCBI Taxonomy refs (TaxIDs): 470
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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