Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: pneumonia [ICD11:
CA40 
];
bacteremia [ICD11:
MA15.0 ];
meningitis [ICD11:
1D01 ];
urinary tract infections (UTI) [ICD11:
GC08 ];
infection due to Acinetobacter baumannii [ICD11:
XN8LS ]
The structure was elucidated in this paperNCBI PubMed ID: 21463855Publication DOI: 10.1016/j.carres.2011.03.024Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: decastro
unina.it
Institutions: Division of Structural Biochemistry, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Borstel, Germany, Università di Napoli Federico II, Dipartimento di Chimica Organica e Biochimica, Complesso Universitario Monte Sant’Angelo, Napoli, Italy
The structures of the capsular polysaccharides (CPSs) of the two clinical isolates Acinetobacter baumannii SMAL and MG1 were elucidated. Hot phenol/water extractions of the dry biomasses, followed by enzymatic digestions and repeated ultracentrifugations led to the isolation of polysaccharides that were negative in Western blot analysis utilizing an anti-lipid A antibody, thus proving that they were not the LPS O-antigens but CPSs. Their structures were established on the basis of NMR spectroscopy and GC-MS analyses. The A. baumannii MG1 CPS consisted of a linear aminopolysaccharide with acyl substitution heterogeneity at the N-4 amino group of QuipN4N: 4)-α-D-GlcpNAc-(1→4)-α-L-GalpNAcA-(1→3)-β-D-QuipNAc4NR-(1→ R=3-hydroxybutyrryl or acetyl. The repeating unit of the CPS produced by strain SMAL is a pentasaccharide, already reported for the O-antigen moiety from A. baumannii strain ATCC 17961: (formula, see text).
Acinetobacter baumannii, capsular polysaccharide, structural analysis, Western blot
Structure type: polymer chemical repeating unit
Location inside paper: abstract, p. 977, fig.7b
Compound class: CPS, O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130648,IEDB_135813,IEDB_136906,IEDB_137340,IEDB_137472,IEDB_137473,IEDB_140529,IEDB_141794,IEDB_141807,IEDB_142488,IEDB_146664,IEDB_151528,IEDB_151531,IEDB_167069,IEDB_190606,IEDB_983931,SB_192,SB_21,SB_7
Methods: 13C NMR, 1H NMR, SDS-PAGE, sugar analysis, deacylation, Western blotting, NMR-1D
Related record ID(s): 26202, 26626, 26628
NCBI Taxonomy refs (TaxIDs): 470Reference(s) to other database(s): GTC:G47927SM, GlycomeDB:
37288
Show glycosyltransferases
NMR conditions: in D2O / 30mM NaOD at 291 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,3 bDGlcp 104.6 73.2 75.7 68.8 73.9 65.1
3,2 Ac
3 bDGalpN 105.5 52.2 83.3 67.4 74.8 61.2
4,2 Ac
4,3 Ac
4 bDGlcpN3NA 103.9 56.5 57.8 72.2 77.8 175.9
6,2 Ac
6 bDGlcpN 103.5 56.4 75.5 69.7 75.9 60.8
aDGalp 97.8 67.6 79.6 78.0 69.3 70.3
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,3 bDGlcp 4.64 3.38 3.49 3.60 3.64 3.77-4.04
3,2 Ac
3 bDGalpN 4.53 3.06 3.69 4.16 3.67 3.76-3.79
4,2 Ac
4,3 Ac
4 bDGlcpN3NA 4.76 2.52 2.67 3.36 3.69 -
6,2 Ac
6 bDGlcpN 4.37 2.61 3.36 3.36 3.44 3.75-3.92
aDGalp 5.03 4.06 4.06 4.47 4.16 3.88-4.11
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,3 bDGlcp 104.6/4.64 73.2/3.38 75.7/3.49 68.8/3.60 73.9/3.64 65.1/3.77-4.04
3,2 Ac
3 bDGalpN 105.5/4.53 52.2/3.06 83.3/3.69 67.4/4.16 74.8/3.67 61.2/3.76-3.79
4,2 Ac
4,3 Ac
4 bDGlcpN3NA 103.9/4.76 56.5/2.52 57.8/2.67 72.2/3.36 77.8/3.69
6,2 Ac
6 bDGlcpN 103.5/4.37 56.4/2.61 75.5/3.36 69.7/3.36 75.9/3.44 60.8/3.75-3.92
aDGalp 97.8/5.03 67.6/4.06 79.6/4.06 78.0/4.47 69.3/4.16 70.3/3.88-4.11
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,3 | bDGlcp | 4.64 | 3.38 | 3.49 | 3.60 | 3.64 | 3.77
4.04 |
| 3,2 | Ac | |
| 3 | bDGalpN | 4.53 | 3.06 | 3.69 | 4.16 | 3.67 | 3.76
3.79 |
| 4,2 | Ac | |
| 4,3 | Ac | |
| 4 | bDGlcpN3NA | 4.76 | 2.52 | 2.67 | 3.36 | 3.69 |
|
| 6,2 | Ac | |
| 6 | bDGlcpN | 4.37 | 2.61 | 3.36 | 3.36 | 3.44 | 3.75
3.92 |
| | aDGalp | 5.03 | 4.06 | 4.06 | 4.47 | 4.16 | 3.88
4.11 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,3 | bDGlcp | 104.6 | 73.2 | 75.7 | 68.8 | 73.9 | 65.1 |
| 3,2 | Ac | |
| 3 | bDGalpN | 105.5 | 52.2 | 83.3 | 67.4 | 74.8 | 61.2 |
| 4,2 | Ac | |
| 4,3 | Ac | |
| 4 | bDGlcpN3NA | 103.9 | 56.5 | 57.8 | 72.2 | 77.8 | 175.9 |
| 6,2 | Ac | |
| 6 | bDGlcpN | 103.5 | 56.4 | 75.5 | 69.7 | 75.9 | 60.8 |
| | aDGalp | 97.8 | 67.6 | 79.6 | 78.0 | 69.3 | 70.3 |
|
There is only one chemically distinct structure:
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Found 1 record.
Displayed record 1
