Houliston RS, Vinogradov E, Dzieciatkowska M, Li J, St Michael F, Karwaski MF, Brochu D, Jarrell HC, Parker CT, Yuki N, Mandrell RE, Gilbert M Lipooligosaccharide of Campylobacter jejuni: Similarity with multiple types of mammalian glycans beyond gangliosides Journal of Biological Chemistry286(14) (2011)
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The structure was elucidated in this paper NCBI PubMed ID:21257763 Publication DOI:10.1074/jbc.M110.181750 Journal NLM ID:2985121R Publisher: Baltimore, MD: American Society for Biochemistry and Molecular Biology Correspondence: michel.gilbertnrc-cnrc.gc.ca Institutions: From the Institute for Biological Sciences, National Research Council, Ottawa, Ontario K1A 0R6, Canada
Campylobacter jejuni is well known for synthesizing ganglioside mimics within the glycan component of its lipooligosaccharide (LOS), which have been implicated in triggering Guillain-Barre syndrome. We now confirm that this pathogen is capable of synthesizing a much broader spectrum of host glycolipid/glycoprotein mimics within its LOS. P blood group and paragloboside (lacto-N-neotetraose) antigen mimicry is exhibited by RM1221, a strain isolated from a poultry source. RM1503, a gastroenteritis-associated strain, expresses lacto-N-biose and sialyl-Lewis c units, the latter known as the pancreatic tumor-associated antigen, DU-PAN-2 (or LSTa). C. jejuni GC149, a Guillain-Barre syndrome-associated strain, expresses an unusual sialic acid-containing hybrid oligosaccharide with similarity to both ganglio and P(k) antigens and can, through phase variation of its LOS biosynthesis genes, display GT1a or GD3 ganglioside mimics. We show that the sialyltransferase CstII and the galactosyltransferase CgtD are involved in the synthesis of multiple mimic types, with LOS structural diversity achieved through evolving allelic substrate specificity.
Methods: 13C NMR, 1H NMR, methylation, NMR-2D, GC-MS, sugar analysis, 31P NMR, NMR-1D, genetic methods, de-N-O-acylation, LC-MS, CE Biosynthesis and genetic data: genetic data, substrate specificity of CgtD and CstII Synthetic data: chemoenzymatic Comments, role: phase variation cgtA(orf5)- GD3 ganglioside mimic, core oligosaccharide from Campylobacter jejuni GC149 serotype HS:31
Related record ID(s): 26226, 26647, 26648, 26650, 26651 NCBI Taxonomy refs (TaxIDs):197 Reference(s) to other database(s): GTC:G87515QH Show glycosyltransferases
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