Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
The structure was elucidated in this paperNCBI PubMed ID: 1813107Publication DOI: 10.1016/0008-6215(91)89023-9Journal NLM ID: 0043535Publisher: Elsevier
Institutions: Max-Planck-Institut für Immunobiologie, Freiburg-Zähringen, Germany
The capsular polysaccharide (K98 antigen) of E. coli O7:K98:H6 contains rhamnose, glucuronic acid, and acetate in the molar ratios 3:1:0.6. Methylation analysis, oligosaccharide analysis, and 1D- and 2D-n.m.r. spectroscopy revealed the polysaccharide to be a glucuronic acid-substituted rhamnan with the structure [formula; see text] Of the 3-linked rhamnose residues, approximately 60% are O-acetylated at position 2.
Structure type: polymer chemical repeating unit
Location inside paper: p.249, table 3
Compound class: CPS, K-antigen
Contained glycoepitopes: IEDB_115136,IEDB_133754,IEDB_136105,IEDB_140630,IEDB_2116141,IEDB_225177,IEDB_423153,IEDB_885823
Methods: 13C NMR, 1H NMR, methylation, GLC-MS, NMR-2D, sugar analysis, ELISA, carboxyl reduction, de-O-acetylation
Comments, role: O-deacetylated CPS
Related record ID(s): 116384
NCBI Taxonomy refs (TaxIDs): 2162916Reference(s) to other database(s): GTC:G25225DX, GlycomeDB:
6107
Show glycosyltransferases
NMR conditions: in D2O at 343 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
2,3 aLRhap 102.8 70.8 78.4 72.6 ? ?
2,2 bDGlcpA 104.8 74.2 76.4 72.8 ? ?
2 aLRhap 101.5 79.6 77.6 72.6 ? ?
aLRhap 101.7 78.9 70.8 73.3 ? ?
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
2,3 aLRhap 4.98 4.10 3.76 3.54 ? ?
2,2 bDGlcpA 4.52 3.30 3.45 3.58 ? -
2 aLRhap 5.20 4.16 3.88 3.60 ? ?
aLRhap 5.12 4.01 3.84 3.43 ? ?
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
2,3 aLRhap 102.8/4.98 70.8/4.10 78.4/3.76 72.6/3.54 ?/? ?/?
2,2 bDGlcpA 104.8/4.52 74.2/3.30 76.4/3.45 72.8/3.58 ?/?
2 aLRhap 101.5/5.20 79.6/4.16 77.6/3.88 72.6/3.60 ?/? ?/?
aLRhap 101.7/5.12 78.9/4.01 70.8/3.84 73.3/3.43 ?/? ?/?
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 2,3 | aLRhap | 4.98 | 4.10 | 3.76 | 3.54 | ? | ? |
| 2,2 | bDGlcpA | 4.52 | 3.30 | 3.45 | 3.58 | ? |
|
| 2 | aLRhap | 5.20 | 4.16 | 3.88 | 3.60 | ? | ? |
| | aLRhap | 5.12 | 4.01 | 3.84 | 3.43 | ? | ? |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 2,3 | aLRhap | 102.8 | 70.8 | 78.4 | 72.6 | ? | ? |
| 2,2 | bDGlcpA | 104.8 | 74.2 | 76.4 | 72.8 | ? | ? |
| 2 | aLRhap | 101.5 | 79.6 | 77.6 | 72.6 | ? | ? |
| | aLRhap | 101.7 | 78.9 | 70.8 | 73.3 | ? | ? |
|
![](nmr_drawer.py?width=750&height=130&nucleus=C&rotate_labels=false&spectrum=[{"s":102.8,"l":"1","c":[0,0,225]},{"s":70.8,"l":"2","c":[0,0,225]},{"s":78.4,"l":"3","c":[0,0,225]},{"s":72.6,"l":"4","c":[0,0,225]},{"s":104.8,"l":"1","c":[230,0,30]},{"s":74.2,"l":"2","c":[230,0,30]},{"s":76.4,"l":"3","c":[230,0,30]},{"s":72.8,"l":"4","c":[230,0,30]},{"s":101.5,"l":"1","c":[0,165,0]},{"s":79.6,"l":"2","c":[0,165,0]},{"s":77.6,"l":"3","c":[0,165,0]},{"s":72.6,"l":"4","c":[0,165,0]},{"s":101.7,"l":"1","c":[120,0,225]},{"s":78.9,"l":"2","c":[120,0,225]},{"s":70.8,"l":"3","c":[120,0,225]},{"s":73.3,"l":"4","c":[120,0,225]}])
The spectrum also has 8 signals at unknown positions (not plotted). |
There is only one chemically distinct structure:
Found 
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