Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
NCBI PubMed ID: 21871432Publication DOI: 10.1016/j.ab.2011.07.035Journal NLM ID: 0370535Publisher: Academic Press
Correspondence: M. Nitz <mnitz
chem.utoronto.ca>
Institutions: Department of Chemistry, University of Toronto, Toronto, Ontario, Canada M5S 3H6
Fluorescent flow cytometry has become the method of choice for interrogation of bacterial populations at the single-cell level. However, limitations of this technique include issues of dynamic range, spectral overlap, photobleaching, and overall low signal intensity due to the small size of bacteria. The recent development of mass cytometry allows single-cell analysis with the resolution of inductively coupled plasma mass spectrometry, facilitating multiparametric analysis. Using a combination of a metal-based membrane stain and lectins conjugated to lanthanide-chelating polymers, we demonstrate that individual Escherichia coli cells can be differentiated based on their cell surface polysaccharides using mass cytometry. The model E. coli system involves evaluation of three different surface polysaccharides using element-tagged concanavalin A and wheat germ agglutinin lectins. Finally, this technique enabled experiments designed to follow the export of O-antigen substituted lipopolysaccharide in a conditional mutant. These studies revealed that the culture responds as a uniform population and that lipopolysaccharide export is approximately 10 times faster than the logarithmic bacterial doubling time.
O-antigen, Escherichia coli, lectins, flow cytometry, mass cytometry
Structure type: polymer chemical repeating unit
Location inside paper: p.4 fig.2, O9a
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_115576,IEDB_130701,IEDB_136104,IEDB_140116,IEDB_141111,IEDB_141830,IEDB_143632,IEDB_144983,IEDB_152206,IEDB_164174,IEDB_164175,IEDB_164176,IEDB_174840,IEDB_241100,IEDB_76933,IEDB_983930,SB_136,SB_196,SB_197,SB_44,SB_67,SB_72
Methods: fluorescent flow cytometry
Biological activity: serological data
Related record ID(s): 26268, 26883
NCBI Taxonomy refs (TaxIDs): 1010797Reference(s) to other database(s): GTC:G54439QN, GlycomeDB:
25319
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There is only one chemically distinct structure:
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