The O-polysaccharide of Vibrio cholerae O43 was studied using chemical analyses, triflic acid solvolysis and 2D NMR spectroscopy, including (1)H/(1)H COSY, TOCSY, NOESY and (1)H/(13)C gradient-selected HSQC experiments. The following structure of the tetrasaccharide repeating unit of the polysaccharide was established: →3)-β-D-Quip4NAcyl-(1→3)-α-D-GalpNAcA-(1→4)-α-D-GalpNAc-(1→3)-α-D-QuipNAc-(1→ where D-QuiNAc stands for 2-acetamido-2,6-dideoxy-D-glucose, D-Qui4NAcyl for 4-(N-acetyl-L-allothreonyl)amino-4,6-dideoxy-D-glucose and D-GalNAcA for 2-acetamido-2-deoxy-D-galacturonic acid.

Lipopolysaccharide, bacterial polysaccharide structure, Vibrio cholerae, 4-amino-4, O-antigen gene cluster, L-allothreonine, 6-D-dideoxy-D-glucose

13C NMR data:
missing...
1H NMR data:
Linkage	Residue	H1	H2	H3	H4	H5	H6
4,2	Ac	-	2.00-2.07
4	aDGalpNA	5.17	4.22	3.97	4.33	4.52	-
2	Ac	-	2.00-2.07
	xDGalN-ol	3.57-3.61	4.12	4.00	3.79	3.99	3.68-3.68

There is only one chemically distinct structure:

SVGMWSMILES3D molIonize

 

CC(=O)N[C@@H](CO)[C@@H](O)[C@@H](O[C@H]1O[C@H](C(=O)O)[C@H](O)[C@H](O)[C@H]1NC(C)=O)[C@H](O)CO

440.402 g/mol (C₁₆H₂₈N₂O₁₂)