Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: pneumonia [ICD11:
CA40 
];
urinary tract infections (UTI) [ICD11:
GC08 ];
liver abscess [ICD11:
DB90.0 , ICD11:
SA04 ];
infection due to Klebsiella pneumoniae [ICD11:
XN741 ]
NCBI PubMed ID: 21478151Publication DOI: 10.1074/jbc.M111.222091Journal NLM ID: 2985121RPublisher: Baltimore, MD: American Society for Biochemistry and Molecular Biology
Correspondence: kfhua
niu.edu.tw; shwu
gate.sinica.edu.tw
Institutions: Institute of Biological Chemistry, Academia Sinica, Taipei 115, Institute of Biotechnology, National Ilan University, I-Lan 260, Department of Horticulture, National Taiwan University, Taipei 106, Genomics Research Center, Academia Sinica, Taipei 115, Department of Microbiology, National Taiwan University, Taipei 100, Department of Internal Medicine, National Taiwan University Hospital, Taipei 100, Taiwan
The active components of a primary pyrogenic liver abscess (PLA) Klebsiella pneumoniae in stimulating cytokine expression in macrophages are still unclear. The capsular polysaccharide (CPS) of PLA K. pneumoniae is important in determining clinical manifestations, and we have shown that it consists of repeating units of the trisaccharide (→3)-β-D-Glc-(1→4)-[2,3-(S)-pyruvate]-β-D-GlcA-(1→4)-α-L-Fuc-(1→) and has the unusual feature of extensive pyruvation of glucuronic acid and acetylation of C2-OH or C3-OH of fucose. We demonstrated that PLA K. pneumoniae CPS induces secretion of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) by macrophages through Toll-like receptor 4 (TLR4) and that this effect was lost when pyruvation and O-acetylation were chemically destroyed. Furthermore, expression of TNF-α and IL-6 in PLA K. pneumoniae CPS-stimulated macrophages was shown to be regulated by the TLR4/ROS/PKC-δ/NF-κB, TLR4/PI3-kinase/AKT/NF-κB, and TLR4/MAPK signaling pathways.
capsular polysaccharide, Klebsiella pneumoniae, inflammation, innate immunity, carbohydrate structure, pathogen-associated molecular pattern, Toll-like Receptors (TLR)
Structure type: structural motif or average structure
Location inside paper: p.2011, fig.1E
Compound class: CPS
Contained glycoepitopes: IEDB_115136,IEDB_136045,IEDB_140630,IEDB_142488,IEDB_142489,IEDB_144562,IEDB_146664,IEDB_152214,IEDB_174333,IEDB_423153,IEDB_983931,SB_192,SB_86
Methods: 13C NMR, 1H NMR, NMR-2D, partial acid hydrolysis, GC-MS, sugar analysis, Western blotting, NMR-1D, methanolysis, serological methods, genetic methods, colorimetry, statistical analysis
Biological activity: interaction between TLR4 and PLA K. pneumoniae CPS
Comments, role: analytical product; CPS after partial acid hydrolysis. Error: there is one extra Glc residue in the figure.
3D data: molecular modeling
Related record ID(s): 26397
NCBI Taxonomy refs (TaxIDs): 573
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There is only one chemically distinct structure:
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