Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Yersinia enterocolitica [ICD11:
XN91V 
]
The structure was elucidated in this paperNCBI PubMed ID: 22925771Publication DOI: 10.1016/j.carres.2012.06.002Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: abeczala
fz-borstel.de (A. Beczała)
Institutions: Division of Structural Biochemistry, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Borstel, Germany
The genus Yersinia represents Gram-negative bacteria that are widely distributed in the environment and possess lipopolysaccharide (LPS) as a major molecule on the surface of the bacterial envelope. LPS is composed of an anchoring lipid, termed lipid A, and a polysaccharide part which not only determines the bacterial serotype but may also serve as a bacteriophage receptor. Here we present the structure of the O-polysaccharide (O-PS) of Yersinia enterocolitica O:50 phage PhiR1-37-sensitive strain 3229. The structural characterization was performed utilizing 1D and 2D nuclear magnetic resonance spectroscopy and chemical analyses. The structure of the O-PS biological repeating unit was identified as →2)-α-L-Rhap-(1→3)-α-L-FucpNAc-(1→3)-α-L-FucpNAc-(1→3)-β-D-GlcpNAc-(1→.
NMR, LPS structure, Yersinia enterocolitica O:50, phage ΦR1-37
Structure type: polymer biological repeating unit ; n=1-4
Location inside paper: p.100
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_135813,IEDB_136105,IEDB_137340,IEDB_141807,IEDB_143253,IEDB_151531,IEDB_225177,IEDB_885823
Methods: 13C NMR, 1H NMR, methylation, GLC-MS, NMR-2D, ESI-ICR-MS, DOC-PAGE, composition analysis, NMR-1D
NCBI Taxonomy refs (TaxIDs): 630Reference(s) to other database(s): GTC:G62079NM
Show glycosyltransferases
NMR conditions: in D2O at 300 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,3,3 aLRhap 95.64 79.74 70.49 72.74 67.93 17.65
3,3,2 Ac
3,3 aLFucpN 98.72 48.15 73.10 67.72 67.44 16.18
3,2 Ac
3 aLFucpN 94.13 47.94 72.85 67.30 67.39 16.23
2 Ac
bDGlcpN 103.49 56.48 79.51 69.16 76.49 61.49
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,3,3 aLRhap 5.07 3.99 3.70 3.29 3.89 1.29
3,3,2 Ac - 2.05
3,3 aLFucpN 5.02 4.35 3.90 3.99 4.33 1.20
3,2 Ac - 2.04
3 aLFucpN 5.01 4.34 3.76 4.04 4.35 1.19
2 Ac - 1.99
bDGlcpN 4.64 3.92 3.67 3.53 3.46 3.75-3.91
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,3,3 aLRhap 95.64/5.07 79.74/3.99 70.49/3.70 72.74/3.29 67.93/3.89 17.65/1.29
3,3,2 Ac NMR TSV error 2: unequal length of 13C and 1H datasets
3,3 aLFucpN 98.72/5.02 48.15/4.35 73.10/3.90 67.72/3.99 67.44/4.33 16.18/1.20
3,2 Ac NMR TSV error 2: unequal length of 13C and 1H datasets
3 aLFucpN 94.13/5.01 47.94/4.34 72.85/3.76 67.30/4.04 67.39/4.35 16.23/1.19
2 Ac NMR TSV error 2: unequal length of 13C and 1H datasets
bDGlcpN 103.49/4.64 56.48/3.92 79.51/3.67 69.16/3.53 76.49/3.46 61.49/3.75-3.91
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,3,3 | aLRhap | 5.07 | 3.99 | 3.70 | 3.29 | 3.89 | 1.29 |
| 3,3,2 | Ac |
| 2.05 | |
| 3,3 | aLFucpN | 5.02 | 4.35 | 3.90 | 3.99 | 4.33 | 1.20 |
| 3,2 | Ac |
| 2.04 | |
| 3 | aLFucpN | 5.01 | 4.34 | 3.76 | 4.04 | 4.35 | 1.19 |
| 2 | Ac |
| 1.99 | |
| | bDGlcpN | 4.64 | 3.92 | 3.67 | 3.53 | 3.46 | 3.75
3.91 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,3,3 | aLRhap | 95.64 | 79.74 | 70.49 | 72.74 | 67.93 | 17.65 |
| 3,3,2 | Ac | |
| 3,3 | aLFucpN | 98.72 | 48.15 | 73.10 | 67.72 | 67.44 | 16.18 |
| 3,2 | Ac | |
| 3 | aLFucpN | 94.13 | 47.94 | 72.85 | 67.30 | 67.39 | 16.23 |
| 2 | Ac | |
| | bDGlcpN | 103.49 | 56.48 | 79.51 | 69.16 | 76.49 | 61.49 |
|
There is only one chemically distinct structure:
Found 
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