Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Burkholderia mallei [ICD11:
XN6Y3 
];
infection due to Pseudomonas mallei [ICD11:
XN52E ];
infection due to Burkholderia pseudomallei [ICD11:
XN3LD ];
infection due to Pseudomonas pseudomallei [ICD11:
XN8AA ]
NCBI PubMed ID: 22912938Publication DOI: 10.3389/fcimb.2012.00108Journal NLM ID: 101585359Publisher: Lausanne: Frontiers Media SA
Correspondence: pbrett
southalabama.edu
Institutions: Department of Microbiology and Immunology, University of South Alabama Mobile, AL, USA
Burkholderia pseudomallei and Burkholderia mallei, the etiologic agents of melioidosis and glanders, respectively, cause severe disease in humans and animals and are considered potential agents of biological warfare and terrorism. Diagnosis and treatment of infections caused by these pathogens can be challenging and, in the absence of chemotherapeutic intervention, acute disease is frequently fatal. At present, there are no human or veterinary vaccines available for immunization against these emerging/re-emerging infectious diseases. One of the long term objectives of our research, therefore, is to identify and characterize protective antigens expressed by B. pseudomallei and B. mallei and use them to develop efficacious vaccine candidates. Previous studies have demonstrated that the 6-deoxy-heptan capsular polysaccharide (CPS) expressed by these bacterial pathogens is both a virulence determinant and a protective antigen. Consequently, this carbohydrate moiety has become an important component of the various subunit vaccines that we are currently developing in our laboratory. In the present study, we describe a reliable method for isolating CPS antigens from O-polysaccharide (OPS) deficient strains of B. pseudomallei; including a derivative of the select agent excluded strain Bp82. Utilizing these purified CPS samples, we also describe a simple procedure for covalently linking these T-cell independent antigens to carrier proteins. In addition, we demonstrate that high titer IgG responses can be raised against the CPS component of such constructs. Collectively, these approaches provide a tangible starting point for the development of novel CPS-based glycoconjugates for immunization against melioidosis and glanders.
capsular polysaccharide, immunization, Burkholderia pseudomallei, vaccine, glycoconjugate, Burkholderia mallei
Structure type: homopolymer
Location inside paper: p.109
Trivial name: 6-deoxy-heptan CPS
Compound class: CPS, O-polysaccharide, O-antigen
Methods: 13C NMR, 1H NMR, PCR, Western blotting, genetic methods, conjugation
Biological activity: Immune responses against CPS-based glycoconjugates
Biosynthesis and genetic data: genetic data
NCBI Taxonomy refs (TaxIDs): 13373,
28450Reference(s) to other database(s): GTC:G09650IH, GlycomeDB:
3383
Show glycosyltransferases
There is only one chemically distinct structure:
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(previously named: Pseudomonas mallei)