Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: gastroenteritis [ICD11:
1A40.0 
];
infection due to Salmonella enterica [ICD11:
XN5VC ]
NCBI PubMed ID: 23028318Publication DOI: 10.1371/journal.ppat.1002918Journal NLM ID: 101238921Publisher: San Francisco, CA: Public Library of Science
Correspondence: ajbaumler
ucdavis.edu
Institutions: Department of Medical Microbiology and Immunology, School of Medicine, University of California at Davis, Davis, California, USA
Intestinal inflammation changes the luminal habitat for microbes through mechanisms that have not been fully resolved. We noticed that the FepE regulator of very long O-antigen chain assembly in the enteric pathogen Salmonella enterica serotype Typhimurium (S. Typhimurium) conferred a luminal fitness advantage in the mouse colitis model. However, a fepE mutant was not defective for survival in tissue, resistance to complement or resistance to polymyxin B. We performed metabolite profiling to identify changes in the luminal habitat that accompany S. Typhimurium-induced colitis. This analysis suggested that S. Typhimurium-induced colitis increased the luminal concentrations of total bile acids. A mutation in fepE significantly reduced the minimal inhibitory concentration (MIC) of S. Typhimurium for bile acids in vitro. Oral administration of the bile acid sequestrant cholestyramine resin lowered the concentrations of total bile acids in colon contents during S. Typhimurium infection and significantly reduced the luminal fitness advantage conferred by the fepE gene in the mouse colitis model. Collectively, these data suggested that very long O-antigen chains function in bile acid resistance of S. Typhimurium, a property conferring a fitness advantage during luminal growth in the inflamed intestine.
O-antigen, Salmonella enterica, Typhimurium, colitis
Structure type: suggested polymer biological repeating unit ; n=1-15
Location inside paper: p.e1002918, 1
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_127517,IEDB_130701,IEDB_135509,IEDB_135513,IEDB_135514,IEDB_135611,IEDB_136093,IEDB_136105,IEDB_136775,IEDB_136906,IEDB_137472,IEDB_137486,IEDB_141794,IEDB_144983,IEDB_151528,IEDB_152206,IEDB_190606,IEDB_225177,IEDB_885823,IEDB_983930,SB_44,SB_67,SB_7,SB_72
Methods: SDS-PAGE, serological methods, genetic methods, statistical analysis
NCBI Taxonomy refs (TaxIDs): 90371Reference(s) to other database(s): GTC:G42214IC
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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(later renamed to: Salmonella enterica ssp. enterica sv. Typhimurium IR715)