Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: gastroenteritis [ICD11:
1A40.0 
];
bloody diarrhea [ICD11:
ME05.1 , ICD11:
SA55 ];
hemorrhagic colitis (HC) [ICD11:
1A40.0 ];
infection due to Escherichia coli [ICD11:
XN6P4 ]
The structure was elucidated in this paperNCBI PubMed ID: 23103511Publication DOI: 10.1016/j.carres.2012.09.017Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: C. De Castro <decastro
unina.it>
Institutions: Universita di Napoli Federico II, Dipartimento di Scienze Chimiche, Complesso Universitario Monte Sant'Angelo, Via Cintia 4, 80126 Napoli, Italy
Two cyclic forms of the Enterobacterial Common Antigen were isolated from Escherichia coli O157:H(-). These antigenic determinants were purified from the biomass through extensive chemical, enzymatic and chromatographic procedures whereas MALDI MS spectrometry indicated their cyclic nature with a polymerization degree of 4 or 5. The two species, denoted as ECA(CYC-4) and ECA(CYC-5), were assigned by NMR and showed no further substitution with other appendages such as acetyl groups as usually described for similar cyclic antigens from other Enterobacteriaceae.
NMR, enterobacterial common antigen, ECA, MALDI
Structure type: cyclic polymer repeating unit ; n=4-5
Location inside paper: abstract, p.30, ECACYC4 and ECACYC5
Compound class: enterobacterial common antigen
Contained glycoepitopes: IEDB_137340,IEDB_141807,IEDB_151531
Methods: 13C NMR, 1H NMR, NMR-2D, GC-MS, SDS-PAGE, sugar analysis, MALDI-TOF MS
Comments, role: Escherichia coli O157:H(-) stx1-, stx2-strain A2CK, NCID, Sofia, Bilgaria. NMR data of the tetrameric form ECACYC4.
NCBI Taxonomy refs (TaxIDs): 183192Reference(s) to other database(s): GTC:G63996VH
Show glycosyltransferases
NMR conditions: in D2O at 308 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
4,4,4 Ac 175.4 23.2
4,4 aDFucp4N 102.0 68.8 72.7 50.8 67.7 16.6
4,2 Ac 176.6 23.3
4 bDManpNA 99.6 54.2 73.4 79.3 78.7 175.9
2 Ac 175.2 23.3
aDGlcpN 94.2 54.3 70.5 78.9 71.4 61.2
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
4,4,4 Ac - 2.07
4,4 aDFucp4N 5.12 3.80 4.07 4.32 4.25 1.04
4,2 Ac - 2.07
4 bDManpNA 4.86 4.53 4.06 3.76 3.82 -
2 Ac - 2.01
aDGlcpN 4.97 3.98 3.82 3.77 4.03 3.76-3.84
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
4,4,4 Ac 23.2/2.07
4,4 aDFucp4N 102.0/5.12 68.8/3.80 72.7/4.07 50.8/4.32 67.7/4.25 16.6/1.04
4,2 Ac 23.3/2.07
4 bDManpNA 99.6/4.86 54.2/4.53 73.4/4.06 79.3/3.76 78.7/3.82
2 Ac 23.3/2.01
aDGlcpN 94.2/4.97 54.3/3.98 70.5/3.82 78.9/3.77 71.4/4.03 61.2/3.76-3.84
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 4,4,4 | Ac |
| 2.07 | |
| 4,4 | aDFucp4N | 5.12 | 3.80 | 4.07 | 4.32 | 4.25 | 1.04 |
| 4,2 | Ac |
| 2.07 | |
| 4 | bDManpNA | 4.86 | 4.53 | 4.06 | 3.76 | 3.82 |
|
| 2 | Ac |
| 2.01 | |
| | aDGlcpN | 4.97 | 3.98 | 3.82 | 3.77 | 4.03 | 3.76
3.84 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 4,4,4 | Ac | 175.4 | 23.2 | |
| 4,4 | aDFucp4N | 102.0 | 68.8 | 72.7 | 50.8 | 67.7 | 16.6 |
| 4,2 | Ac | 176.6 | 23.3 | |
| 4 | bDManpNA | 99.6 | 54.2 | 73.4 | 79.3 | 78.7 | 175.9 |
| 2 | Ac | 175.2 | 23.3 | |
| | aDGlcpN | 94.2 | 54.3 | 70.5 | 78.9 | 71.4 | 61.2 |
|
There is only one chemically distinct structure:
Found 
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