Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: glanders [ICD11:
1B92 
, ICD11:
XN6Y3 ];
infection due to Burkholderia mallei [ICD11:
XN6Y3 ];
infection due to Pseudomonas mallei [ICD11:
XN52E ];
infection due to Burkholderia pseudomallei [ICD11:
XN3LD ];
infection due to Pseudomonas pseudomallei [ICD11:
XN8AA ]
The structure was elucidated in this paperNCBI PubMed ID: 22221792Publication DOI: 10.1016/j.carres.2011.12.011Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: C. Heiss <cheiss
ccrc.uga.edu>; M.N. Burtnick <mburtnick
southalabama.edu>
Institutions: Complex Carbohydrate Research Center, The University of Georgia, Athens, GA 30602, USA
Capsular polysaccharides (CPSs) were isolated from O-polysaccharide deficient strains of Burkholderia mallei and Burkholderia pseudomallei using a modified hot phenol/water extraction procedure. Glycosyl composition, methylation, MALDI-TOF MS analyses as well as (1)H NMR spectroscopy including COSY, TOCSY, NOESY, HMBC and HSQC experiments identified the presence of two distinct CPS antigens in the samples exhibiting the following structures: This study confirms the ability of B. mallei to express a 6-deoxy-heptan CPS and represents the first report of a mannan CPS being expressed by these bacterial pathogens.
structure, capsular polysaccharide, Burkholderia pseudomallei, Burkholderia mallei, protective antigen NMR
Structure type: homopolymer
Location inside paper: abstract
Trivial name: 6-deoxy-heptan CPS
Compound class: CPS, O-polysaccharide, O-antigen
Methods: 13C NMR, 1H NMR, methylation, NMR-2D, GC-MS, MALDI-TOF MS, composition analysis, NMR-1D
Related record ID(s): 28390
NCBI Taxonomy refs (TaxIDs): 13373,
28450Reference(s) to other database(s): GTC:G09650IH, GlycomeDB:
3383
Show glycosyltransferases
NMR conditions: in D2O at 323 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6 C7
2 Ac
bD6dmanHepp 98.7 72.0 81.6 71.5 75.5 36.1 61.0
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6 H7
2 Ac
bD6dmanHepp 4.90 5.29 3.96 3.48 3.49 1.76-2.15 3.77
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6 C7/H7
2 Ac
bD6dmanHepp 98.7/4.90 72.0/5.29 81.6/3.96 71.5/3.48 75.5/3.49 36.1/1.76-2.15 61.0/3.77
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 | H7 |
|---|
| 2 | Ac | |
| | bD6dmanHepp | 4.90 | 5.29 | 3.96 | 3.48 | 3.49 | 1.76
2.15 | 3.77 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 | C7 |
|---|
| 2 | Ac | |
| | bD6dmanHepp | 98.7 | 72.0 | 81.6 | 71.5 | 75.5 | 36.1 | 61.0 |
|
There is only one chemically distinct structure:
Found 
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(previously named: Pseudomonas mallei BM1987)