Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
The structure was elucidated in this paperPublication DOI: 10.1016/0008-6215(92)80088-IJournal NLM ID: 0043535Publisher: Elsevier
Institutions: School of Pharmaceutical Sciences, Rhodes University, Grahamstown 6140 South Africa
The structure of the capsular antigen of E. coli O8:K102:H− was investigated by methylation analysis, β-elimination of the methylated polys
Structure type: polymer chemical repeating unit
Location inside paper: p.202, table II, structure 2 (DP)
Compound class: K-antigen
Contained glycoepitopes: IEDB_130651,IEDB_136044,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_144987,IEDB_151528,IEDB_190606,IEDB_742247,SB_165,SB_166,SB_187,SB_195,SB_31,SB_62,SB_7,SB_88
Methods: 13C NMR, 1H NMR, methylation, GLC-MS, NMR-2D, sugar analysis, Li/ethylenediamine degradation
Comments, role: E. coli 08:K102:H- (No. 6CBlO/l); the trisaccharide repeating unit of the lithium-degraded polymer (DP)
Related record ID(s): 116862
NCBI Taxonomy refs (TaxIDs): 562Reference(s) to other database(s): GTC:G95676PX, GlycomeDB:
6294
Show glycosyltransferases
NMR conditions: in D2O at 313 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
4,4 bDGalp 105.20 71.50 83.05 69.40 75.70 ?
4 aDGalp 101.20 69.70 70.60 79.30 71.10 ?
bDGalp 105.45 71.70 73.20 78.10 75.90 ?
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
4,4 bDGalp 4.67 3.78 3.88 4.15 3.73 ?
4 aDGalp 4.97 3.94 4.03 4.29 4.43 3.71-3.84
bDGalp 4.71 3.65 3.77 4.05 3.78 ?
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
4,4 bDGalp 105.20/4.67 71.50/3.78 83.05/3.88 69.40/4.15 75.70/3.73 ?/?
4 aDGalp 101.20/4.97 69.70/3.94 70.60/4.03 79.30/4.29 71.10/4.43 ?/3.71-3.84
bDGalp 105.45/4.71 71.70/3.65 73.20/3.77 78.10/4.05 75.90/3.78 ?/?
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 4,4 | bDGalp | 4.67 | 3.78 | 3.88 | 4.15 | 3.73 | ? |
| 4 | aDGalp | 4.97 | 3.94 | 4.03 | 4.29 | 4.43 | 3.71
3.84 |
| | bDGalp | 4.71 | 3.65 | 3.77 | 4.05 | 3.78 | ? |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 4,4 | bDGalp | 105.20 | 71.50 | 83.05 | 69.40 | 75.70 | ? |
| 4 | aDGalp | 101.20 | 69.70 | 70.60 | 79.30 | 71.10 | ? |
| | bDGalp | 105.45 | 71.70 | 73.20 | 78.10 | 75.90 | ? |
|
![](nmr_drawer.py?width=750&height=130&nucleus=C&rotate_labels=false&spectrum=[{"s":105.2,"l":"1","c":[0,0,225]},{"s":71.5,"l":"2","c":[0,0,225]},{"s":83.05,"l":"3","c":[0,0,225]},{"s":69.4,"l":"4","c":[0,0,225]},{"s":75.7,"l":"5","c":[0,0,225]},{"s":101.2,"l":"1","c":[230,0,30]},{"s":69.7,"l":"2","c":[230,0,30]},{"s":70.6,"l":"3","c":[230,0,30]},{"s":79.3,"l":"4","c":[230,0,30]},{"s":71.1,"l":"5","c":[230,0,30]},{"s":105.45,"l":"1","c":[0,165,0]},{"s":71.7,"l":"2","c":[0,165,0]},{"s":73.2,"l":"3","c":[0,165,0]},{"s":78.1,"l":"4","c":[0,165,0]},{"s":75.9,"l":"5","c":[0,165,0]}])
The spectrum also has 3 signals at unknown positions (not plotted). |
There is only one chemically distinct structure:
Found 
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