Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Burkholderia [ICD11:
XN01M 
]
The structure was elucidated in this paperNCBI PubMed ID: 22115719Publication DOI: 10.1016/j.carres.2011.10.038Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: A. Molinaro <molinaro
unina.it>
Institutions: Dipartimento di Chimica Organica e Biochimica, Universita di Napoli Federico II, via Cintia 4, 80126 Napoli, Italy, Department of Biomolecular Chemistry, Leibniz Institute for Natural Product Research and Infection Biology, HKI, Beutenbergstr. 11a, 07745 Jena, Germany
Two different polysaccharides were isolated and identified from the lipopolysaccharide fraction of endofungal bacterium Burkholderia sp. HKI-402 (B4). The complete structure was elucidated by chemical analysis and 2D NMR spectroscopy as the following: [formula: see text].
lipopolysaccharide O-antigen, Burkholderia rhizoxinica, Endosymbiontic bacteria
Structure type: polymer chemical repeating unit
Location inside paper: abstract, p.96
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_133754,IEDB_135813,IEDB_135849,IEDB_136105,IEDB_137340,IEDB_141807,IEDB_151531,IEDB_225177,IEDB_885823
Methods: 13C NMR, 1H NMR, NMR-2D, GC-MS, SDS-PAGE, chemical analysis, NMR-1D
Related record ID(s): 28633
NCBI Taxonomy refs (TaxIDs): 36773Reference(s) to other database(s): GTC:G46744WV
Show glycosyltransferases
NMR conditions: in D2O at 298 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,2 aLRhap 102.7 70.2 80.4 71.4 69.7 17.3
3 aLRhap 100.1 80.6 70.4 72.7 69.6 17.3
2 Ac
bDGlcpN 102.2 56.4 82.0 69.1 76.2 61.2
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,2 aLRhap 4.94 4.33 3.91 3.57 3.82 1.36
3 aLRhap 5.14 3.85 3.94 3.53 4.08 1.33
2 Ac
bDGlcpN 4.87 3.92 3.63 3.61 3.55 3.84-4.01
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,2 aLRhap 102.7/4.94 70.2/4.33 80.4/3.91 71.4/3.57 69.7/3.82 17.3/1.36
3 aLRhap 100.1/5.14 80.6/3.85 70.4/3.94 72.7/3.53 69.6/4.08 17.3/1.33
2 Ac
bDGlcpN 102.2/4.87 56.4/3.92 82.0/3.63 69.1/3.61 76.2/3.55 61.2/3.84-4.01
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,2 | aLRhap | 4.94 | 4.33 | 3.91 | 3.57 | 3.82 | 1.36 |
| 3 | aLRhap | 5.14 | 3.85 | 3.94 | 3.53 | 4.08 | 1.33 |
| 2 | Ac | |
| | bDGlcpN | 4.87 | 3.92 | 3.63 | 3.61 | 3.55 | 3.84
4.01 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,2 | aLRhap | 102.7 | 70.2 | 80.4 | 71.4 | 69.7 | 17.3 |
| 3 | aLRhap | 100.1 | 80.6 | 70.4 | 72.7 | 69.6 | 17.3 |
| 2 | Ac | |
| | bDGlcpN | 102.2 | 56.4 | 82.0 | 69.1 | 76.2 | 61.2 |
|
There is only one chemically distinct structure:
Found 
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