Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
The structure was elucidated in this paperPublication DOI: 10.1134/S0026261712020154Journal NLM ID: 0376652Publisher: Moskva: Izdatelstvo Nauka
Correspondence: varbanets
serv.imv.kiev.ua
Institutions: Zabolotnyi Institute of Microbiology and Virology, National Academy of Sciences of Ukraine,ul. Zabolotnogo 154, Kiev, 252143 Ukraine
The lipopolysaccharide (LPS) of Escherichia coli M-17 was isolated, studied, chemically identified, and shown to be an apyrogenic compound of low toxicity. Investigation of the effect of this LPS on T- and B-lymphocytes suggests that it can be used as a mitogen in blast transformation reactions, as it is only slightly less active than the commercial preparation. Double immunodiffusion in agar by Ouchterlony revealed that the LPS of E. coli M-17 in a homologous system exhibited an antigenic activity and did not interact with the antisera against representatives of other Enterobacteriaceae species (Budvicia aquatica, Rahnella aquatilis, and Pragia fontium) in serological cross-reactions. Mild acid hydrolysis yielded the following structural components of the lipopolysaccharide molecule: lipid A, core oligosaccharide, and O-specific polysaccharide. The structure of the O-specific polysaccharide determined using the data on the monosaccharide composition and the 1H and 13NMR spectra was found to be typical of representatives of the E. coli serogroup O2: [formula: see text].
Lipopolysaccharide, structure, Escherichia coli, O-specific polysaccharide, biological activity
Structure type: polymer chemical repeating unit
Location inside paper: abstract, p.328
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_133754,IEDB_135813,IEDB_136105,IEDB_137340,IEDB_141807,IEDB_151531,IEDB_225177,IEDB_885823
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, GLC, mild acid hydrolysis, NMR-1D, serological methods
Biological activity: biological activity data of the LPS
Comments, role: published polymerization frame was shifted for conformity with other records
NCBI Taxonomy refs (TaxIDs): 2067429Reference(s) to other database(s): GTC:G75832AT, GlycomeDB:
28094
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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