Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
The structure was elucidated in this paperNCBI PubMed ID: 2983984Publication DOI: 10.1111/j.0014-2956.1985.00601.xJournal NLM ID: 0107600Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Institutions: Max-Planck-Institut für Immunbiologie, Stübeweg 51, D-7800 Freiburg‐Zähringen, Federal Republic of Germany
The chemical structure of the K52 antigenic capsular polysaccharide (K52 antigen) of Escherichia coli O4:K52:H- was elucidated by composition, nuclear magnetic resonance spectroscopy, methylation, periodate oxidation before and after graded acid hydrolysis and by oligosaccharide analysis. The polysaccharide consists of a backbone of α-galactose units interlinked between C1 and C3 by phosphodiester bridges. This poly(α-galactosyl-phosphate) is substituted at C2 of each galactose unit by β-fructofuranose residues. About 80% of the galactose units are O-acetylated at C4 and about 10% of the fructose units are both O-acetylated and O-propionylated at C1. The K52 polysaccharide has an average molecular mass of 34 kDa, thus consisting of approximately 65 fructosyl-galactosyl-phosphate repeating units.
Structure type: polymer chemical repeating unit
Location inside paper: table 4, p.608, fig.5
Compound class: K-antigen
Contained glycoepitopes: IEDB_136906,IEDB_137472,IEDB_141794,IEDB_145001,IEDB_151528,IEDB_190606,SB_7
Methods: 13C NMR, 1H NMR, GC-MS
Comments, role: E. coli A 103 (O4:K52:H-); deacylated CPS
Related record ID(s): 122878
NCBI Taxonomy refs (TaxIDs): 562Reference(s) to other database(s): GTC:G04885IT, GlycomeDB:
16604
Show glycosyltransferases
NMR conditions: in D2O at 303(C) K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
0,2 bDFruf 63.8 104.8 79.1 76.1 82.1 63.8
0 aDGalp 97.3 68.9 75.7 70.4 73.0 62.1
P
1H NMR data:
missing...
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 0,2 | bDFruf | 63.8 | 104.8 | 79.1 | 76.1 | 82.1 | 63.8 |
| 0 | aDGalp | 97.3 | 68.9 | 75.7 | 70.4 | 73.0 | 62.1 |
| | P | |
|
There is only one chemically distinct structure:
Found 
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