Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
];
infection due to Klebsiella pneumoniae [ICD11:
XN741 ]
The structure was elucidated in this paperNCBI PubMed ID: 22169179Publication DOI: 10.1016/j.carres.2011.11.019Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: E. Vinogradov <evguenii.vinogradov
nrc.ca>
Institutions: National Institute of Child Health and Human Development, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD, USA, Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada N1G 2W1, Institute of Immunology and Experimental Therapy, Polish Academy of Science, Wroclaw, Poland
O-specific polysaccharides of Gram-negative bacteria are synthesized by two different mechanisms: polymerization of the pre-formed O-repeating unit or sequential addition of the monosaccharides to the growing polysaccharide chain. In the second case, growth of the polymer can be further subdivided into two groups depending on the presence or absence of a special monosaccharide or non-sugar substituent that terminates the glycan. A family of polymannose O-polysaccharides provides prototypes for the chain terminating process. Polysaccharides of Klebsiella pneumoniae O3, Hafnia alvei PCM 1223, and Escherichia coli O9 have the same penta-mannose repeating unit. E. coli O9a has tetra-mannose repeat and this structure can be produced by mutants of E. coli O9. The mechanism of biosynthesis of H. alvei 1223 O-polysaccharide has not been reported. Here we show that all above polysaccharides contain the same modification at the non-reducing end; presence of a methyl phosphate group at O-3 of α-mannopyranose, that serves as the signal for termination of the chain elongation.
LPS, O-specific polysaccharide, Klebsiella, Hafnia, Klebsiella pneumoniae, methyl phosphate
Structure type: polymer chemical repeating unit
Location inside paper: p.188
Trivial name: mannose homopolysaccharide
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_115576,IEDB_130701,IEDB_136104,IEDB_140116,IEDB_141111,IEDB_141795,IEDB_141830,IEDB_143632,IEDB_144983,IEDB_152206,IEDB_153756,IEDB_164174,IEDB_164175,IEDB_164176,IEDB_164480,IEDB_174840,IEDB_241100,IEDB_76933,IEDB_983930,SB_136,SB_196,SB_197,SB_44,SB_67,SB_72
Methods: 13C NMR, 1H NMR, sugar analysis, 31P NMR, mild acid hydrolysis, GPC, MR-2D
Comments, role: published polymerization frame was shifted for conformity with other records.
Related record ID(s): 27225, 28436, 28438
NCBI Taxonomy refs (TaxIDs): 1010797,
573,
569Reference(s) to other database(s): GTC:G87424QV, GlycomeDB:
668, CCSD:
47538
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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