Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: urinary tract infections (UTI) [ICD11:
GC08 
]
NCBI PubMed ID: 22282517Publication DOI: 10.1099/mic.0.055210-0Journal NLM ID: 0376646Publisher: Washington, DC: Kluwer Academic/Plenum Publishers
Correspondence: wanglei
nankai.edu.cn; yknirel
gmail.com
Institutions: TEDA School of Biological Sciences and Biotechnology, Nankai University, 23 Hongda Street, TEDA, 300457 Tianjin, PR China
Enterobacteria of the genus Providencia are opportunistic human pathogens associated with urinary tract and wound infections, as well as enteric diseases. The lipopolysaccharide (LPS) O antigen confers major antigenic variability upon the cell surface and is used for serotyping of Gram-negative bacteria. Recently, Providencia O antigen structures have been extensively studied, but no data on the location and organization of the O antigen gene cluster have been reported. In this study, the four Providencia genome sequences available were analysed, and the putative O antigen gene cluster was identified in the polymorphic locus between the cpxA and yibK genes. This finding provided the necessary information for designing primers, and cloning and sequencing the O antigen gene clusters from five more Providencia alcalifaciens strains. The gene functions predicted in silico were in agreement with the known O antigen structures; furthermore, annotation of the genes involved in the three-step synthesis of GDP-colitose (gmd, colD and colC) was supported by cloning and biochemical characterization of the corresponding enzymes. In one strain (P. alcalifaciens O39), no polysaccharide product of the gene cluster in the cpxA-yibK locus was found, and hence genes for synthesis of the existing O antigen are located elsewhere in the genome. In addition to the putative O antigen synthesis genes, homologues of wza, wzb, wzc and (in three strains) wzi, required for the surface expression of capsular polysaccharides, were found upstream of yibK in all species except Providencia rustigianii, suggesting that the LPS of these species may be attributed to the so-called K LPS (K(LPS)). The data obtained open a way for development of a PCR-based typing method for identification of Providencia isolates.
Lipopolysaccharide, Providencia alcalifaciens, O-antigen gene cluster, O-Polysaccharide structure
Structure type: polymer chemical repeating unit
Location inside paper: p.1028
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_115136,IEDB_134623,IEDB_136044,IEDB_136105,IEDB_137340,IEDB_137472,IEDB_137485,IEDB_140630,IEDB_141794,IEDB_141807,IEDB_144983,IEDB_151531,IEDB_152206,IEDB_190606,IEDB_225177,IEDB_423153,IEDB_885823,IEDB_983930,SB_165,SB_166,SB_187,SB_195,SB_44,SB_7,SB_72,SB_88
Methods: 13C NMR, 1H NMR, NMR-2D, PCR, DNA sequencing, SDS-PAGE, glycosyltransferase assays, 31P NMR, ESI-MS, genetic methods, HPLC
Biosynthesis and genetic data: genetic data
Comments, role: published polymerization frame was shifted for conformity with other records
Related record ID(s): 27267, 28531, 28532, 28533, 28534, 28535, 28536, 28537, 28538, 28539
NCBI Taxonomy refs (TaxIDs): 588Reference(s) to other database(s): GTC:G95526HJ, GlycomeDB:
25892
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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