Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: diarrhea [ICD11:
ME05.1 
, ICD11:
SA55 ]
NCBI PubMed ID: 23163869Publication DOI: 10.1111/1574-695X.12002Journal NLM ID: 9315554Publisher: Elsevier
Correspondence: olga.ovchinnikova
gmail.com
Institutions: TEDA School of Biological Sciences and Biotechnology, Nankai University, Tianjin, China
The O-polysaccharide chain of the lipopolysaccharide (O-antigen) on the bacterial cell surface is one of the most structurally variable cell components and serves as a basis for serotyping of Gram-negative bacteria, including human opportunistic pathogens of the genus Providencia. In this work, the O-antigen of Providencia alcalifaciens O40 was obtained by mild acid degradation of the isolated lipopolysaccharide and studied by chemical methods and high-resolution NMR spectroscopy. The following structure of the O-polysaccharide was established: -4)-b-D-Quip3NFo-(1-3)-a-D-Galp-(1-3)-b-D-GlcpA-(1-3)-b-D-GalpNAc-(1- where GlcA stands for glucuronic acid and Qui3NFo for 3,6-dideoxy-3-formamidoglucose. The O40-antigen was found to be structurally and serologically related to the O-antigens of P. alcalifaciens O5 and Providencia stuartii O18. The O40-antigen gene cluster between cpxA and yibK was sequenced, and the gene functions were predicted in silico. In agreement with the O-polysaccharide structure established, the genes for the synthesis of dTDP-D-Qui3NFo, UDP-D-Gal, UDP-D-GlcA, and UDP-D-GalNAc as well as those encoding three glycosyltransferases, flippase (Wzx), and O-antigen polymerase (Wzy) were recognized. In addition, homologues of wza, wzb, and wzc genes, which are required for the surface expression of capsular polysaccharides, were found within the gene cluster, suggesting that the O-polysaccharide studied is a part of the capsule-related form of the lipopolysaccharide called K(LPS).
Lipopolysaccharide, Providencia, Providencia alcalifaciens, 3, O-antigen gene cluster, O-Polysaccharide structure, 6-dideoxy-3-formamidoglucose
Structure type: polymer chemical repeating unit
Location inside paper: p.386, fig.4, 3
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_115136,IEDB_130648,IEDB_137340,IEDB_137473,IEDB_1391961,IEDB_140630,IEDB_141584,IEDB_141807,IEDB_151531,IEDB_423153,IEDB_885822
Methods: 13C NMR, 1H NMR, NMR-2D, DNA sequencing, sugar analysis, ESI-MS, mild acid hydrolysis, serological methods
Biosynthesis and genetic data: genetic data
Related record ID(s): 27268, 28540
NCBI Taxonomy refs (TaxIDs): 588Reference(s) to other database(s): GTC:G71840FF, GlycomeDB:
27452
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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