CSDB: Search results

Taxonomic group: bacteria / Firmicutes (Phylum: Firmicutes)
Host organism: Homo sapiens
Associated disease: neonatal septicemia [ICD11: KA60

, Life stage: neonatal]; meningitis [ICD11: 1D01

]; infection due to Streptococcus [ICD11: XN3NM

]

NCBI PubMed ID: 22634296
Publication DOI: 10.1016/j.vaccine.2012.05.006
Journal NLM ID: 8406899
Publisher: Elsevier
Correspondence: spincus

chnola-research.org
Institutions: Research Institute for Children, Children's Hospital, New Orleans, LA 70118, United States, Institute for Biological Sciences, National Research Council of Canada, Ottawa, ON, Canada K1A 0R6, Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL 35294, United States, Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294, United States, Copenhagen Center for Glycomics (CCG), Departments of Cellular and Molecular Medicine and Dentistry, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3, DK-2200, Copenhagen N, Denmark

Group B streptococcus (GBS) is a major cause of neonatal sepsis and meningitis. Despite aggressive campaigns using antenatal prophylactic antibiotic therapy, infections continue. Developing an effective maternal vaccine is a public health priority. Antibody (Ab) to the capsular polysaccharide (CPS) is considered the dominant 'protective' immune mediator. Here we study the fine specificity and potential host reactivity of a panel of well-characterized murine monoclonal Abs against the type III CPS by examining the binding of the Abs to intact and neuraminidase-digested GBS, purified CPS, synthetic carbohydrate structures, and cells. The results showed marked differences in the fine specificity among these mAbs to a single carbohydrate structure. Cross-reactions with synthetic GD3 and GT3 carbohydrates, representing structures found on surfaces of neural and developing cells, were demonstrated using carbohydrate array technology. The anti-CPS(III) mAbs did not react with cells expressing GD3 and GT3, nor did mAbs specific for the host carbohydrates cross-react with GBS, raising questions about the physiological relevance of this cross-reaction. But in the process of these investigations, we serendipitously demonstrated cross-reactions of some anti-CPS(III) mAbs with antigens, likely carbohydrates, found on human leukocytes. These studies suggest caution in the development of a maternal vaccine to prevent infection by this important human pathogen.

capsular polysaccharide, group B Streptococcus, cross-reaction, monoclonal Ab, fine specificity, carbohydrate array

Structure type: oligomer
Location inside paper: p.4853, fig.3, 170
Aglycon: (CH2)3NH2
Contained glycoepitopes: IEDB_115015,IEDB_130646,IEDB_135813,IEDB_136044,IEDB_136794,IEDB_137340,IEDB_137472,IEDB_140108,IEDB_140122,IEDB_141794,IEDB_141807,IEDB_142354,IEDB_142489,IEDB_146100,IEDB_149135,IEDB_149174,IEDB_150933,IEDB_151531,IEDB_190606,IEDB_241119,IEDB_241120,IEDB_423120,SB_115,SB_116,SB_131,SB_165,SB_166,SB_170,SB_171,SB_172,SB_187,SB_195,SB_30,SB_39,SB_68,SB_7,SB_84,SB_86,SB_88

Methods: ELISA, biological assays, serological methods, binding assays, immunofluorescence analyses
Biological activity: fine specificity and cross-reactions of a panel of mAbs to the type III CPS of group B streptococcus

Related record ID(s): 27283, 28575, 28576, 28577, 28578, 28579, 28580, 28581, 28583, 28584
NCBI Taxonomy refs (TaxIDs): 1319
Reference(s) to other database(s): GTC:G30348HB
Show glycosyltransferases

There is only one chemically distinct structure:

CC(=O)N[C@@H]1[C@@H](O[C@H]2O[C@H](C)[C@H](O)[C@H](O)[C@H]2O)[C@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@H](O[C@]3(C(=O)O)C[C@H](O)[C@@H](NC(C)=O)[C@H]([C@H](O)[C@H](O)CO)O3)[C@H]2O)[C@@H](COS(=O)(=O)O)O[C@H]1O

SVG MW SMILES 3D mol Ionize