CSDB: Search results

Taxonomic group: bacteria / Firmicutes (Phylum: Firmicutes)
Associated disease: infection due to Clostridium difficile [ICD11: XN0SE

]

NCBI PubMed ID: 22560631
Publication DOI: 10.1016/j.carres.2012.02.002
Journal NLM ID: 0043535
Publisher: Elsevier
Correspondence: S.M. Logan <susan.logan

nrc-cnrc.gc.ca>
Institutions: National Research Council-Institute for Biological Sciences, 100 Sussex Drive, Ottawa, ON, Canada K1A 0R6

Whole-cell high-resolution magic angle spinning (HR-MAS) NMR was employed to survey the surface polysaccharides of a group of clinical and environmental isolates of Clostridium difficile. Results indicated that a highly conserved surface polysaccharide profile among all strains studied. Multiple additional peaks in the anomeric region were also observed which prompted further investigation. Structural characterization of the isolated surface polysaccharides from two strains confirmed the presence of the conserved water soluble polysaccharide originally described by Ganeshapillai et al. which was composed of a hexaglycosyl phosphate repeat consisting of [→6)-β-D-Glcp-(1-3)-β-D-GalpNAc-(1-4)-α-D-Glcp-(1-4)-[β-D-Glcp(1-3]-β-D-GalpNAc-(1-3)-α-D-Manp-(1-P→]. In addition, analysis of phenol soluble polysaccharides revealed a similarly conserved lipoteichoic acid (LTA) which could be detected on whole cells by HR-MAS NMR. Conventional NMR and mass spectrometry analysis indicated that the structure of this LTA consisted of the repeat unit [→6)-α-D-GlcpNAc-(1-3)-[→P-6]-α-D-GlcpNAc-(1-2)-D-GroA] where GroA is glyceric acid. The repeating units were linked by a phosphodiester bridge between C-6 of the two GlcNAc residues (6-P-6). A minor component consisted of GlcpN-(1-3) instead of GlcpNAc-(1-3) in the repeat unit. Through a 6-6 phosphodiester bridge this polymer was linked to →6)-β-D-Glcp-(1-6)-β-D-Glcp-(1-6)-β-D-Glcp-(1-1)-Gro, with glycerol (Gro) substituted by fatty acids. This is the first report of the utility of HR-MAS NMR in the examination of surface carbohydrates of Gram positive bacteria and identification of a novel LTA structure from Clostridium difficile.

capsular polysaccharide, lipoteichoic acid, Clostridium difficile, lipocarbohydrate, High-resolution magic angle spinning (HRMAS) NMR

Structure type: polymer chemical repeating unit
Location inside paper: abstract, p.65, PSI
Trivial name: cell-surface polysaccharide, pentaglycosyl phosphate repeating unit cell-surface polysaccharide, rhamnoglucan PSI, PS1, cell-surface polysaccharide PS-I, PSI, PSI repeating unit
Compound class: cell wall polysaccharide, lipoteichoic acid
Contained glycoepitopes: IEDB_136105,IEDB_142488,IEDB_144998,IEDB_145002,IEDB_146664,IEDB_189515,IEDB_189517,IEDB_225177,IEDB_232583,IEDB_232584,IEDB_232585,IEDB_885823,IEDB_983931,SB_192

Methods: 13C NMR, 1H NMR, de-O-acylation, sugar analysis, 31P NMR, HF treatment, CE-MS/MS, HR-MAS NMR
3D data: molecular modeling

Related record ID(s): 27293, 28605, 28606, 28607, 28608, 28609, 28610
NCBI Taxonomy refs (TaxIDs): 1496
Reference(s) to other database(s): GTC:G60087JR, GlycomeDB:36857
Show glycosyltransferases

There is only one chemically distinct structure:

*O[C@H]1[C@H](C)O[C@@H](O[C@@H]2[C@@H](O)[C@H](O[C@@H]3[C@@H](CO)O[C@H](O[C@@H]4[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]4OP(*)(=O)O)[C@H](O)[C@H]3O[C@@H]3O[C@@H](C)[C@H](O)[C@@H](O)[C@H]3O)O[C@H](CO)[C@H]2O)[C@H](O)[C@@H]1O

SVG MW SMILES 3D mol Ionize