Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: neonatal meningitis [ICD11:
KA65.4 
, Life stage: neonatal];
neonatal necrotising enterocolitis [ICD11:
KB88 , Life stage: neonatal]
NCBI PubMed ID: 22447597Publication DOI: 10.1128/AEM.07825-11Journal NLM ID: 7605801Publisher: American Society for Microbiology
Correspondence: Lei Wang <wanglei
nankai.edu.cn>
Institutions: Nankai University, Tianjin, China
The Gram-negative bacteria Cronobacter sakazakii is an emerging foodborne pathogen causing severe invasive infections in neonates. Variation in the O antigen lipopolysaccharide in the outer membrane provides the basis of Gram-negative bacteria serotyping. The O antigen serotyping scheme for C. sakazakii, which includes seven serotypes (O1 to O7), has been recently established, and the O antigen gene clusters and specific primers for three C. sakazakii serotypes (O1, O2, and O3) have been characterized. In this study, the C. sakazakii O4, O5, O6, and O7 O antigen gene clusters were sequenced and gene functions were predicted on the basis of homology. C. sakazakii O4 shared a similar O antigen gene cluster with E. coli O103. The general features and anomalies of all seven C. sakazakii O antigen gene clusters were evaluated and the relationship between O antigen structures and their gene clusters were investigated. Serotype-specific genes for O4 to O7 were identified and a molecular serotyping method for all C. sakazakii O serotypes, multiplex PCR assay, was developed by screening against 136 strains of C. sakazakii and closely related species. The sensitivity of PCR-based serotyping method was determined to be 0.01 ng genomic DNAs and 10(3) CFU/ml of strains. This study completes the elucidation of C. sakazakii O antigen genetics and provides a molecular method suitable for identification of C. sakazakii O1 to O7 strains.
Lipopolysaccharide, structure, PCR, O-serotype, O-antigen gene cluster, Cronobacter
Structure type: polymer chemical repeating unit
Location inside paper: p.3969, fig.1C
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_135813,IEDB_137340,IEDB_141807,IEDB_151531
Methods: serological methods, genetic methods
Biosynthesis and genetic data: genetic data
Related record ID(s): 27329, 28654, 28655, 28656
NCBI Taxonomy refs (TaxIDs): 28141Reference(s) to other database(s): GTC:G39677CL
Show glycosyltransferases
There is only one chemically distinct structure:
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(previously named: Enterobacter sakazakii O7)