Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: gastroenteritis [ICD11:
1A40.0 
];
infection due to Salmonella enterica [ICD11:
XN5VC ]
The structure was elucidated in this paperNCBI PubMed ID: 23030432Publication DOI: 10.1021/ja307467pJournal NLM ID: 7503056Publisher: American Chemical Society
Correspondence: markus.aebi
micro.biol.ethz.ch; beme
ethz.ch
Institutions: Physical Chemistry, ETH Zurich , Wolfgang-Pauli Str. 10, 8093 Zurich Switzerland
While structural information on biomolecules is mainly obtained from purified in vitro samples, NMR can also be applied in the context of entire cells or organisms. The present study describes maturation processes in living Salmonella enterica serovar Typhimurium, a prevalent cause for human gastroenteritis. In our physiological study, we follow the composition of the O-antigen on the outer bacterial membrane with high-resolution MAS NMR spectroscopy. We detect and characterize an evolution of the O-antigen composition, in particular of the O-acetylation state of the O-antigen, a factor that can play an important role in vaccine development.
O-antigen, O-acetylation, Salmonella enterica, outer membrane, Typhimurium, MAS NMR spectroscopy
Structure type: polymer chemical repeating unit ; n=70-100
Location inside paper: p.17513, chart 1
Compound class: O-antigen
Contained glycoepitopes: IEDB_127517,IEDB_130701,IEDB_135509,IEDB_135513,IEDB_135514,IEDB_135611,IEDB_136093,IEDB_136105,IEDB_136775,IEDB_136906,IEDB_137472,IEDB_137486,IEDB_141794,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_152206,IEDB_190606,IEDB_225177,IEDB_885823,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_67,SB_7,SB_72
Methods: 1H NMR, NMR-2D, HR-MAS NMR
Comments, role: mutant strain without functional acetyltransferase gene (M2012: SL1344 oafA::Tn10); published polymerization frame was shifted for conformity with other records.
Related record ID(s): 27366
NCBI Taxonomy refs (TaxIDs): 216597Reference(s) to other database(s): GTC:G15961TR
Show glycosyltransferases
NMR conditions: in D2O at 302(H) K
[as TSV]
13C NMR data:
missing...
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,4,3 aXAbep 5.09 4.03 1.98 3.87 4.11 1.18
3,4 aDManp 5.33 4.00 ? ? 3.98 3.81
3 aLRhap 5.05 4.07 3.97 3.55 3.93 1.33
4 aDGlcp
aDGalp 5.17 3.91 3.94 4.07 4.09 3.71
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,4,3 | aXAbep | 5.09 | 4.03 | 1.98 | 3.87 | 4.11 | 1.18 |
| 3,4 | aDManp | 5.33 | 4.00 | ? | ? | 3.98 | 3.81 |
| 3 | aLRhap | 5.05 | 4.07 | 3.97 | 3.55 | 3.93 | 1.33 |
| 4 | aDGlcp | |
| | aDGalp | 5.17 | 3.91 | 3.94 | 4.07 | 4.09 | 3.71 |
|
There is only one chemically distinct structure:
Found 
report error