Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: urinary tract infections (UTI) [ICD11:
GC08 
];
meningitis [ICD11:
1D01 ];
septicemia [ICD11:
MA15.Y ];
infection due to Acinetobacter baumannii [ICD11:
XN8LS ]
The structure was elucidated in this paperPublication DOI: 10.1002/ejoc.200901396Journal NLM ID: 9805750Publisher: Wiley-VCH
Correspondence: decastro
unina.it
Institutions: Department of Organic Chemistry and Biochemistry, University of Napoli Federico II - Complesso Universitario Monte Sant’Angelo, Via Cinthia 4, 80126 Napoli, Italy
Acinetobacter baumannii is a pathogenic organism that posesses a serious health threat because of the occurrence of the large number of (multi)drug-resistant strains. It can persist for prolonged periods in the hospital environment, infecting debilitated or immune-compromised patients. In this context, the endotoxin portion of the lipopolysaccharide, the lipid A, plays an important role in the pathogenesis of this bacterium, because it triggers the innate immune response and contributes to the inflammation state of the patient. In this study, the complete structure of the lipooligosaccharide has been determined. The saccharide backbone of the molecule was disclosed through chemical and spectroscopic analysis, whereas the lipid A moiety required detailed MS spectrometry and chemical investigations. The oligosaccharide backbone was found to be similar to that of A. baumannii ATCC 19606, although the LOS from the SMAL strain presented an enhanced zwitterionic character. The lipid A moiety comprises a heterogeneous and complex mixture of molecules: it possesses a conserved diphosphorylated disaccharide backbone substituted by three to seven fatty acids. The hexaacylated species appeared as the most abundant, and its chemical features, namely the number and the types of fatty acids, indicates its potential endotoxic activity.
Acinetobacter baumannii, NMR spectroscopy, structure elucidation, mass spectrometry, glycolipids
Structure type: oligomer
Location inside paper: p.1346, fig.1, 3
Compound class: LOS
Contained glycoepitopes: IEDB_130650,IEDB_137473,IEDB_141807,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_151531,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, GC-MS, deacylation, acid hydrolysis, alkaline degradation, ESI-ICR-MS, chemical methods
Comments, role: Product after mild acid hydrolysis of LOS.
Related record ID(s): 27374, 28699, 28701
NCBI Taxonomy refs (TaxIDs): 470Reference(s) to other database(s): GTC:G24180ML
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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