Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
Associated disease: infection due to Bacillus [ICD11:
XN9ZE 
]
NCBI PubMed ID: 7795419Journal NLM ID: 8603310Publisher: Kluwer Academic Publishers
Institutions: Institut für Chemie, Universität für Bodenkultur, Wien, Austria
Preliminary taxonomic characterization of isolate L420-91 has revealed that this organism is closely related to the species Bacillus aneurinolyticus. The bacterium is covered by a squarely arranged crystalline surface layer composed of identical glycoprotein subunits with an apparent molecular mass in the range of 109 kDa. A total carbohydrate content of approximately 3.5% (wt/wt) was determined in the purified surface layer glycoprotein. Glycopeptides were obtained after exhaustive Pronase digestion and purification including gel filtration, ion exchange chromatography and HPLC. From the combined evidence of composition analysis. Smith degradation and nuclear magnetic resonance spectroscopy experiments we propose the following structure for the glycan chain of the surface layer glycoprotein: [formula: see text]
structure, 6-dideoxy-D-galactose, glycan, 3-Acetamido-3, Bacillus, S-layer, D-rhamnose, glycoprotein, Eubacteria, S layer, surface layer (S-layer), glycan structure, ~H and 13C-NMR
Structure type: polymer chemical repeating unit
Location inside paper: 1, abstract
Compound class: S-layer glycoprotein, S-layer glycan
Contained glycoepitopes: IEDB_1394181,IEDB_144827,IEDB_145005,IEDB_145006,IEDB_145010
Methods: 13C NMR, 1H NMR, NMR-2D, SDS-PAGE, Smith degradation, composition analysis, electron microscopy
Comments, role: Parent molecule: S-layer glycoprotein;
Related record ID(s): 4612, 9081
NCBI Taxonomy refs (TaxIDs): 1409Reference(s) to other database(s): GTC:G12009WO, GlycomeDB:
9267, CCSD:
40670, CBank-STR:15828
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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