Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: bacteremia [ICD11:
MA15.0 
];
neonatal necrotising enterocolitis [ICD11:
KB88 , Life stage: neonatal];
neonatal meningitis [ICD11:
KA65.4 , Life stage: neonatal]
The structure was elucidated in this paperNCBI PubMed ID: 22578769Publication DOI: 10.1016/j.carres.2012.02.031Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: yknirel
gmail.com
Institutions: ND Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
The O-specific polysaccharide (O-antigen) was isolated from the lipopolysaccharide of Cronobacter sakazakii G2726 (serotype O3) and studied by sugar analysis, Smith degradation, and (1)H and (13)C NMR spectroscopy. The following structure of the acidic O-polysaccharide was established: [structure: see text]. This structure is closely related to that of the O-polysaccharide of Cronobacter muytjensii 3270, which has the same main chain and differs only in the lack of glucosylation. The O-antigen gene cluster of C. sakazakii G2726 found between the gnd and galF genes was sequenced, and the gene functions were tentatively assigned by similarity to related genes from available databases and taking into account the O-polysaccharide structure.
Lipopolysaccharide, O-specific polysaccharide, bacterial polysaccharide structure, O-antigen gene cluster, Cronobacter sakazakii
Structure type: oligomer
Location inside paper: p.54, table 1
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130648,IEDB_136105,IEDB_137473,IEDB_1391961,IEDB_141584,IEDB_225177,IEDB_885822,IEDB_885823
Methods: 13C NMR, 1H NMR, NMR-2D, DNA sequencing, sugar analysis, acid hydrolysis, GLC, Smith degradation, NMR-1D, GPC
Comments, role: Smith degraded polysaccharide.
Related record ID(s): 27109, 28307
NCBI Taxonomy refs (TaxIDs): 28141
Show glycosyltransferases
NMR conditions: in D2O at 303 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
1,3,4,2 Ac 175.4-175.8 23.2-23.6
1,3,4 aDGalpN 98.2 50.5 68.5 69.7 72.8 62.4
1,3,3 Ac 175.4-175.8 23.2-23.6
1,3 aDQuip3N 96.2 71.7 55.1 77.3 68.3 19.2
1 aLRhap 101.2 68.2 77.3 71.4 69.9 17.9
xDGro 69.8 71.8 63.7
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
1,3,4,2 Ac - 1.98-2.06
1,3,4 aDGalpN 5.28 4.19 3.86 4.02 4.06 3.76-3.76
1,3,3 Ac - 1.98-2.06
1,3 aDQuip3N 5.03 3.58 4.32 3.52 4.22 1.31
1 aLRhap 4.85 4.18 3.85 3.62 3.77 1.35
xDGro 3.50-3.79 3.93 3.60-3.65
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
1,3,4,2 Ac 23.2-23.6/1.98-2.06
1,3,4 aDGalpN 98.2/5.28 50.5/4.19 68.5/3.86 69.7/4.02 72.8/4.06 62.4/3.76-3.76
1,3,3 Ac 23.2-23.6/1.98-2.06
1,3 aDQuip3N 96.2/5.03 71.7/3.58 55.1/4.32 77.3/3.52 68.3/4.22 19.2/1.31
1 aLRhap 101.2/4.85 68.2/4.18 77.3/3.85 71.4/3.62 69.9/3.77 17.9/1.35
xDGro 69.8/3.50-3.79 71.8/3.93 63.7/3.60-3.65
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 1,3,4,2 | Ac |
| 1.98
2.06 | |
| 1,3,4 | aDGalpN | 5.28 | 4.19 | 3.86 | 4.02 | 4.06 | 3.76
3.76 |
| 1,3,3 | Ac |
| 1.98
2.06 | |
| 1,3 | aDQuip3N | 5.03 | 3.58 | 4.32 | 3.52 | 4.22 | 1.31 |
| 1 | aLRhap | 4.85 | 4.18 | 3.85 | 3.62 | 3.77 | 1.35 |
| | xDGro | 3.50
3.79 | 3.93 | 3.60
3.65 | |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 1,3,4,2 | Ac | 175.4
175.8 | 23.2
23.6 | |
| 1,3,4 | aDGalpN | 98.2 | 50.5 | 68.5 | 69.7 | 72.8 | 62.4 |
| 1,3,3 | Ac | 175.4
175.8 | 23.2
23.6 | |
| 1,3 | aDQuip3N | 96.2 | 71.7 | 55.1 | 77.3 | 68.3 | 19.2 |
| 1 | aLRhap | 101.2 | 68.2 | 77.3 | 71.4 | 69.9 | 17.9 |
| | xDGro | 69.8 | 71.8 | 63.7 | |
|
There is only one chemically distinct structure:
Found 
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