Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: diarrhea [ICD11:
ME05.1 
, ICD11:
SA55 ];
infection due to Escherichia coli [ICD11:
XN6P4 ]
NCBI PubMed ID: 22572125Publication DOI: 10.1016/j.carres.2012.02.020Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: G. Widmalm <gw
organ.su.se>
Institutions: Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Stockholm, Sweden
The structure of the repeating unit of the O-antigenic polysaccharide (PS) from Escherichia coli O174 has been determined. Component analysis together with (1)H and (13)C NMR spectroscopy experiments were employed to elucidate the structure. Inter-residue correlations were determined by (1)H,(13)C-heteronuclear multiple-bond correlation and (1)H,(1)H-NOESY experiments. The PS is composed of tetrasaccharide repeating units with the following structure: Cross-peaks of low intensity were present in the NMR spectra consistent with a β-D-GlcpNAc-(1→2)-β-D-GlcpA(1→ structural element at the terminal part of the polysaccharide, which on average is composed of approximately 15 repeating units. Consequently the biological repeating unit has a 3-substituted N-acetyl-d-galactosamine residue at its reducing end.
Lipopolysaccharide, NMR, Escherichia coli, biological repeating unit
Structure type: suggested polymer biological repeating unit
Location inside paper: p.104, fig.4
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_115136,IEDB_130648,IEDB_134627,IEDB_135813,IEDB_136044,IEDB_137340,IEDB_137472,IEDB_137473,IEDB_140630,IEDB_141794,IEDB_141807,IEDB_147450,IEDB_151531,IEDB_190606,IEDB_423153,SB_165,SB_166,SB_187,SB_195,SB_23,SB_24,SB_7,SB_8,SB_88
Methods: 13C NMR, 1H NMR, GLC-MS, NMR-2D, sugar analysis, GLC, NMR-1D
Comments, role: chemical repeat frame is different in the paper
Related record ID(s): 27150, 28337, 28338, 29965, 30040
NCBI Taxonomy refs (TaxIDs): 562Reference(s) to other database(s): GTC:G25286AF
Show glycosyltransferases
There is only one chemically distinct structure:
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