Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
Host organism: Homo sapiens
Associated disease: diarrhea [ICD11:
ME05.1 
, ICD11:
SA55 ];
infection due to Clostridium difficile [ICD11:
XN0SE ]
NCBI PubMed ID: 23597587Publication DOI: 10.1016/j.carres.2013.03.018Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: M.A. Monteiro <monteiro
uoguelph.ca>
Institutions: Department of Chemistry, University of Guelph, Guelph, ON, Canada
Clostridium difficile is the most common cause of antimicrobial-associated diarrhea in humans and may cause death. Previously, we discovered that C. difficile expresses three polysaccharides, named PSI, PSII, and PSIII. It has now been established that PSII is a conserved antigen abundantly present on the cell-surface and biofilm of C. difficile. In contrast, the expression of PSI and PSIII appears to be stochastic processes. In this work, the total chemical synthesis of the PSI pentasaccharide repeating unit carrying a linker at the reducing end, α-L-Rhap-(1→3)-β-D-Glcp-(1→4)-[α-L-Rhap-(1→3)]-α-D-Glcp-(1→2)-α-D-Glcp-(1→O(CH2)5NH2, was achieved by a linear synthesis strategy from four monosaccharide building blocks. The synthesized PSI pentasaccharide was conjugated to a subunit of C. difficile exotoxin B yielding a potential dual C. difficile vaccine. More significantly, sera from healthy horses were shown to contain natural anti-PSI IgG antibodies that detected both the synthetic non-phosphorylated PSI repeat and the native PSI polysaccharide, with a slightly higher recognition of the native PSI polysaccharide.
vaccine, Clostridium difficile, Anti-PSI IgG antibodies, C. difficile PSI polysaccharide, C. difficile Toxin B conjugate
Structure type: polymer chemical repeating unit
Location inside paper: p.15, PS1
Trivial name: cell-surface polysaccharide, pentaglycosyl phosphate repeating unit cell-surface polysaccharide, rhamnoglucan PSI, PS1, cell-surface polysaccharide PS-I, PSI, PSI repeating unit
Compound class: cell wall polysaccharide, lipoteichoic acid
Contained glycoepitopes: IEDB_136105,IEDB_142488,IEDB_144998,IEDB_145002,IEDB_146664,IEDB_189515,IEDB_189517,IEDB_225177,IEDB_232583,IEDB_232584,IEDB_232585,IEDB_885823,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, SDS-PAGE, TLC, ELISA, chemical synthesis, conjugation
Synthetic data: chemical
Comments, role: published polymerization frame was shifted for conformity with other record
Related record ID(s): 29589
NCBI Taxonomy refs (TaxIDs): 1496Reference(s) to other database(s): GTC:G60087JR, GlycomeDB:
36857
Show glycosyltransferases
There is only one chemically distinct structure:
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(later renamed to: Peptoclostridium difficile O27)