E. Katzenellenbogen <katzenel
L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wroclaw, Poland
Mild acid degradation of the lipopolysaccharide of Edwardsiella tarda PCM 1150 afforded an O-polysaccharide, which was isolated by GPC on Sephadex G-50 and studied by sugar and methylation analyses along with 1D and 2D 1H and 13C NMR spectroscopies, including experiments performed in a 9:1 H2O/D2O mixture to detect NH protons and their correlations with CH protons. The O-polysaccharide was found to contain an amide of d-glucuronic acid with l-alanine (d-GlcA6Ala) and the following structure of the branched hexasaccharide repeating unit was established: →4)-β-D-GlcpA6Ala-(1→4)-α-L-Fucp-(1→4)-α-D-Glcp-(1→4)-α-D-Quip-(1→3)-β-D-GlcpNAc-(1→3<←1)-α-D-GalpNAc.
13C NMR, 1H NMR, methylation, GLC-MS, NMR-2D, sugar analysis, acid hydrolysis, GLC
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,4,4,4,6 xLAla? 179.5 52.5 17.9
3,4,4,4 bDGlcpA 104.4 74.3 74.4 77.0 75.5 170.1
3,4,4,3,2 Ac 175.7 23.4
3,4,4,3 aDGalpN 99.8 50.9 68.7 70.1 72.5 62.8
3,4,4 aLFucp 100.4 69.7 74.3 80.8 68.8 16.5
3,4 aDGlcp 100.4 73.1 72.7 77.6 72.1 60.8
3 aDQuip 100.6 73.0 72.7 84.4 68.5 17.8
2 Ac 175.8 23.8
bDGlcpN 99.6 56.3 82.4 70.0 77.4 61.9
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,4,4,4,6 xLAla? - 4.20 1.46
3,4,4,4 bDGlcpA 4.62 3.48 3.63 3.96 3.89 -
3,4,4,3,2 Ac - 2.03
3,4,4,3 aDGalpN 5.16 4.14 3.76 3.79 3.96 3.63-3.78
3,4,4 aLFucp 4.98 3.98 3.98 4.10 4.42 1.28
3,4 aDGlcp 5.03 3.56 3.75 3.57 4.10 3.80-3.83
3 aDQuip 5.00 3.47 3.69 3.22 4.14 1.28
2 Ac - 2.04
bDGlcpN 4.39 3.83 3.67 3.52 3.40 3.76-3.93
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,4,4,4,6 xLAla? 52.5/4.20 17.9/1.46
3,4,4,4 bDGlcpA 104.4/4.62 74.3/3.48 74.4/3.63 77.0/3.96 75.5/3.89
3,4,4,3,2 Ac 23.4/2.03
3,4,4,3 aDGalpN 99.8/5.16 50.9/4.14 68.7/3.76 70.1/3.79 72.5/3.96 62.8/3.63-3.78
3,4,4 aLFucp 100.4/4.98 69.7/3.98 74.3/3.98 80.8/4.10 68.8/4.42 16.5/1.28
3,4 aDGlcp 100.4/5.03 73.1/3.56 72.7/3.75 77.6/3.57 72.1/4.10 60.8/3.80-3.83
3 aDQuip 100.6/5.00 73.0/3.47 72.7/3.69 84.4/3.22 68.5/4.14 17.8/1.28
2 Ac 23.8/2.04
bDGlcpN 99.6/4.39 56.3/3.83 82.4/3.67 70.0/3.52 77.4/3.40 61.9/3.76-3.93
report error
Found 1 record.
Displayed record 1
]; wound infections [ICD11: NF0A.3 
iitd.pan.wroc.pl>