Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: pneumonia [ICD11:
CA40 
];
bacteremia [ICD11:
MA15.0 ];
infection due to Pseudomonas aeruginosa [ICD11:
XN5L6 ]
NCBI PubMed ID: 23664878Publication DOI: 10.1016/j.bmcl.2013.04.051Journal NLM ID: 9107377Publisher: Elsevier
Correspondence: I. Brockhausen <brockhau
queensu.ca>
Institutions: Department of Medicine, Queen's University, Kingston, Ontario, Canada K7L 3N6, Department of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario, Canada K7L 3N6, Carlsberg Laboratory, Copenhagen-V DK-1799, Denmark
Gram negative bacteria have lipopolysaccharides (LPS) that are critical for their survival. LPS molecules are composed of antigenic exopolysaccharide chains (O antigens). We are interested in discovering the enzymes involved in the biosynthesis of O antigens in Pseudomonas aeruginosa. The common polysaccharide antigen contains α-linked d-rhamnose residues. We have now synthesized GDP-d-rhamnose by a convenient synthesis in aqueous solution, and have shown that it can be used without extensive purification as the donor substrate for d-rhamnosyltransferase (WbpZ) from the P. aeruginosa strain PAO1. The availability of this nucleotide sugar preparation allows for characterization of d-rhamnosyltransferases.
Pseudomonas aeruginosa, O-antigens, D-Rha-transferase, WbpZ, GDP-D-Rha
Structure type: polymer chemical repeating unit
Location inside paper: p.3491
Trivial name: common antigen, common polysaccharide antigen, A band polysaccharide, D-rhamnan, repeat unit of the lipopolysaccharide side-chain, A-band polysaccharide (A-PS), A-band polysaccharide
Compound class: O-polysaccharide, O-antigen, LPS, enterobacterial common antigen, CPA
Contained glycoepitopes: IEDB_1394181,IEDB_144831,IEDB_145005,IEDB_145006,IEDB_145010
Methods: 1H NMR, glycosyltransferase assays, ESI-MS, MALDI-MS, Western blotting, chemical synthesis, biochemical methods
Synthetic data: chemoenzymatic
Comments, role: published polymerization frame of a chemical repeating unit was shifted for the uniformity of structures in the database
Related record ID(s): 29809
NCBI Taxonomy refs (TaxIDs): 208964Reference(s) to other database(s): GTC:G80135ZC, GlycomeDB:
25893
Show glycosyltransferases
There is only one chemically distinct structure:
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