Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Burkholderia mallei [ICD11:
XN6Y3 
]
The structure was elucidated in this paperNCBI PubMed ID: 24056008Publication DOI: 10.1016/j.carres.2013.08.013Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: C. Heiss <cheiss
uga.edu>
Institutions: Complex Carbohydrate Research Center, The University of Georgia, Athens, GA 30602, USA, Department of Microbiology and Immunology, University of South Alabama, 5851 USA Drive North, Mobile, AL 36688, USA
O-Polysaccharides (OPS) were isolated from purified Burkholderia pseudomallei and Burkholderia mallei lipopolysaccharides by mild-acid hydrolysis and gel-permeation chromatography. 1-D and 2-D (1)H and (13)C NMR spectroscopy experiments revealed that the OPS antigens were unbranched heteropolymers with the following structures: Collectively, our results demonstrate that the predominant OPS antigens expressed by B. pseudomallei and B. mallei isolates are structurally more complex than previously described and provide evidence that different capping residues are used by these closely related pathogens to terminate chain elongation. Additionally, they confirm that Burkholderia thailandensis and B. pseudomallei express OPS antigens that are essentially identical to one another.
Lipopolysaccharide, structure, O-polysaccharide, Burkholderia pseudomallei, Burkholderia thailandensis, Burkholderia mallei
Structure type: oligomer
Location inside paper: abstract, p.10
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_142488,IEDB_146664,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, gel filtration, NMR-2D, sugar analysis, mild acid hydrolysis, NMR-1D
Comments, role: Burkholderia mallei BM2308 is an ATCC 23344 derivative; terminal part of OPS, part of structure (see RR: 29546,29549)
Related record ID(s): 29196, 29545, 29546, 29548, 29549
NCBI Taxonomy refs (TaxIDs): 243160Reference(s) to other database(s): GTC:G24786ZV
Show glycosyltransferases
NMR conditions: in D2O at 323 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,2 Ac
3,3 Me
3 aL6dTalp 101.1 70.8 76.4 70.3 ? ?
bDGlcp
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,2 Ac
3,3 Me
3 aL6dTalp 5.26 5.25 3.78 3.94 ? ?
bDGlcp
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,2 Ac
3,3 Me
3 aL6dTalp 101.1/5.26 70.8/5.25 76.4/3.78 70.3/3.94 ?/? ?/?
bDGlcp
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,2 | Ac | |
| 3,3 | Me | |
| 3 | aL6dTalp | 5.26 | 5.25 | 3.78 | 3.94 | ? | ? |
| | bDGlcp | |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,2 | Ac | |
| 3,3 | Me | |
| 3 | aL6dTalp | 101.1 | 70.8 | 76.4 | 70.3 | ? | ? |
| | bDGlcp | |
|
![](nmr_drawer.py?width=750&height=130&nucleus=C&rotate_labels=false&spectrum=[{"s":101.1,"l":"1","c":[0,0,225]},{"s":70.8,"l":"2","c":[0,0,225]},{"s":76.4,"l":"3","c":[0,0,225]},{"s":70.3,"l":"4","c":[0,0,225]}])
The spectrum also has 2 signals at unknown positions (not plotted). |
There is only one chemically distinct structure:
Found 
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