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Taxonomic group: bacteria / Proteobacteria (Phylum: Proteobacteria)
Associated disease: infection due to Campylobacter jejuni [ICD11: XN4Q5

]

The structure was elucidated in this paper
NCBI PubMed ID: 23437265
Publication DOI: 10.1371/journal.pone.0056900
Journal NLM ID: 101285081
Publisher: San Francisco, CA: Public Library of Science
Correspondence: akiba

affrc.go.jp
Institutions: Bacterial and Parasitic Disease Research Division, National Institute of Animal Health, Tsukuba, Japan, Analytical Science Division, National Food Research Institute, Tsukuba, Japan, Bioscience Research Education Center, Akita University, Akita, Akita, Japan, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka, Japan

Campylobacter jejuni is the most common bacterium that causes diarrhea worldwide, and chickens are considered the main reservoir of this pathogen. This study investigated the effects of serial truncation of lipooligosaccharide (LOS), a major component of the outer membrane of C. jejuni, on its bile resistance and intestinal colonization ability in chickens. Genes encoding manno-heptose synthetases or glycosyltransferases were inactivated to generate isogenic mutants. Serial truncation of the LOS core oligosaccharide caused a stepwise increase in susceptibilities of two C. jejuni strains, NCTC 11168 and 81-176, to bile acids. Inactivation of hldE, hldD, or waaC caused severe truncation of the core oligosaccharide, which greatly increased the susceptibility to bile acids. Both wild-type strains grew normally in chicken intestinal extracts, whereas the mutants with severe oligosaccharide truncation were not detected 12 h after inoculation. These mutants attained viable bacterial counts in the bile acid-free extracts 24 h after inoculation. The wild-type strain 11-164 was present in the cecal contents at >10(7) CFU/g on 5 days after challenge infection and after this time period, whereas its hldD mutant was present at <10(3) CFU/g throughout the experimental period. Trans-complementation of the hldD mutant with the wild-type hldD allele completely restored the in vivo colonization level to that of the wild-type strain. Mutants with a shorter LOS had higher hydrophobicities. Thus, the length of the LOS core oligosaccharide affected the surface hydrophobicity and bile resistance of C. jejuni as well as its ability to colonize chicken intestines.

Lipooligosaccharide, Campylobacter jejuni, Genes, glycosyltransferases, inner core, outer membrane

Structure type: oligomer
Location inside paper: p.2, fig.1, p.6, fig.2B
Aglycon: lipid A
Compound class: LOS
Contained glycoepitopes: IEDB_120354,IEDB_123890,IEDB_130648,IEDB_130650,IEDB_130658,IEDB_130659,IEDB_130679,IEDB_130683,IEDB_136044,IEDB_136794,IEDB_137472,IEDB_137473,IEDB_140087,IEDB_140088,IEDB_140090,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_146100,IEDB_146664,IEDB_149174,IEDB_150933,IEDB_153778,IEDB_1625329,IEDB_190606,IEDB_2189047,IEDB_983931,SB_116,SB_144,SB_165,SB_166,SB_170,SB_171,SB_172,SB_187,SB_192,SB_195,SB_25,SB_37,SB_39,SB_6,SB_68,SB_7,SB_76,SB_84,SB_88

Methods: PCR, GC-MS, MALDI-TOF MS, genetic methods, SDS-Tricine-PAGE
Biological activity: effects of LOS truncations on antimicrobial resistance, on chick cecum colonization and on cell surface hydrophobicity
Synthetic data: enzymatic in vivo
Comments, role: Campylobacter jejuni 81-176 wild type, 817gmhA mutant.

Related record ID(s): 29207, 29580, 29581, 29582, 29583, 29584
NCBI Taxonomy refs (TaxIDs): 354242
Show glycosyltransferases

There are 5 chemically distinct structures. Please, select:

CC(=O)N[C@H]1[C@H](O[C@H]2[C@@H](CO)O[C@@H](O[C@@H]3[C@@H](CO)O[C@@H](O[C@H]4[C@@H](O)[C@H](O)[C@@H]([C@@H](O)CO)O[C@@H]4O[C@@H]4[C@H](O)[C@@H](O[C@@H]5[C@H](O)CC(O)(C(=O)OC6(C(=O)O)C[C@@H](O)[C@@H](O)[C@@H]([C@H](O)CO)O6)O[C@@H]5[C@H](O)CO)O[C@H]([C@H](CO)OP(=O)(O)OCCN)[C@H]4O[C@@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4O)[C@H](O)[C@H]3O)[C@H](O)[C@H]2O[C@]2(C(=O)O)C[C@H](O)[C@@H](NC(C)=O)[C@H]([C@H](O)[C@H](O)CO)O2)O[C@H](CO)[C@H](O)[C@@H]1O

SVG MW SMILES 3D mol Ionize