Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Brucella melitensis [ICD11:
XN7ZW 
];
infection due to Brucella abortus [ICD11:
XN7A8 ];
infection due to Brucella suis [ICD11:
XN3UP ]
The structure was elucidated in this paperNCBI PubMed ID: 23664729Publication DOI: 10.1016/j.carres.2013.03.021Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: E. Vinogradov <evguenii.vinogradov
nrc.ca>
Institutions: Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA
O-Specific polysaccharides of Brucella contain two antigenic determinants, called A and M. Most of the strains express epitope A with a small amount of epitope M, whereas Brucella melitensis strain 16M expresses longer polymer consisting mostly of M-type epitopes. Proposed explanation was that epitope A is defined by 1-2-linked homopolymer of N-formylperosamine (Rha4NFo), while epitope M is a pentasaccharide with four 2- and one 3-substituted Rha4NFo. We reinvestigated both types of structures by 2D NMR and showed that M-epitope is a tetrasaccharide, missing one of the 2-linked Rha4NFo as compared to the previously proposed structure. Polysaccharide from B. melitensis 16M contains a fragment of 1-2-linked polymer, capped with M-type polymer. Other strains contain one or two M-type units at the non-reducing end of the 1-2-linked O-chain.
NMR, LPS, structure, O-antigens, O-specific, O-specific polysaccharide, Brucella, MS, Brucella melitensis
Structure type: polymer chemical repeating unit
Location inside paper: p.145, table 1
Aglycon: -2)aDRhap4N(1-
Compound class: O-polysaccharide
Methods: 13C NMR, 1H NMR, NMR-2D, GC-MS, sugar analysis, MALDI-TOF MS, NMR-1D, N-acetylation, de-N-acelation
Comments, role: Part of the N-deformylated polysaccharide structure (see RR: 29630). NMR data for B. melitensis 16M.
Related record ID(s): 29248, 29626, 29628, 29629, 29630, 29631
NCBI Taxonomy refs (TaxIDs): 29459,
235,
29461Reference(s) to other database(s): GTC:G87917AJ
Show glycosyltransferases
NMR conditions: in D2O at 298 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
2,3,2 aDRhap4N 101.5 77.5 67.1 54.9 67.0 18.0
2,3 aDRhap4N 101.5 77.6 67.1 54.9 67.4 18.0
2 aDRhap4N 103.0 68.9 77.5 53.3 67.0 18.0
aDRhap4N 101.3 77.5 67.1 54.9 67.0 18.0
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
2,3,2 aDRhap4N 5.26 4.22 4.21 3.29 4.11 1.38
2,3 aDRhap4N 5.19 4.28 4.22 3.29 4.32 1.39
2 aDRhap4N 5.10 4.33 4.21 3.38 4.12 1.39
aDRhap4N 5.29 4.20 4.18 3.26 4.05 1.36
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
2,3,2 aDRhap4N 101.5/5.26 77.5/4.22 67.1/4.21 54.9/3.29 67.0/4.11 18.0/1.38
2,3 aDRhap4N 101.5/5.19 77.6/4.28 67.1/4.22 54.9/3.29 67.4/4.32 18.0/1.39
2 aDRhap4N 103.0/5.10 68.9/4.33 77.5/4.21 53.3/3.38 67.0/4.12 18.0/1.39
aDRhap4N 101.3/5.29 77.5/4.20 67.1/4.18 54.9/3.26 67.0/4.05 18.0/1.36
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 2,3,2 | aDRhap4N | 5.26 | 4.22 | 4.21 | 3.29 | 4.11 | 1.38 |
| 2,3 | aDRhap4N | 5.19 | 4.28 | 4.22 | 3.29 | 4.32 | 1.39 |
| 2 | aDRhap4N | 5.10 | 4.33 | 4.21 | 3.38 | 4.12 | 1.39 |
| | aDRhap4N | 5.29 | 4.20 | 4.18 | 3.26 | 4.05 | 1.36 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 2,3,2 | aDRhap4N | 101.5 | 77.5 | 67.1 | 54.9 | 67.0 | 18.0 |
| 2,3 | aDRhap4N | 101.5 | 77.6 | 67.1 | 54.9 | 67.4 | 18.0 |
| 2 | aDRhap4N | 103.0 | 68.9 | 77.5 | 53.3 | 67.0 | 18.0 |
| | aDRhap4N | 101.3 | 77.5 | 67.1 | 54.9 | 67.0 | 18.0 |
|
There is only one chemically distinct structure:
Found 
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