Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Brucella melitensis [ICD11:
XN7ZW 
];
infection due to Brucella abortus [ICD11:
XN7A8 ];
infection due to Brucella suis [ICD11:
XN3UP ]
The structure was elucidated in this paperNCBI PubMed ID: 23664729Publication DOI: 10.1016/j.carres.2013.03.021Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: E. Vinogradov <evguenii.vinogradov
nrc.ca>
Institutions: Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA
O-Specific polysaccharides of Brucella contain two antigenic determinants, called A and M. Most of the strains express epitope A with a small amount of epitope M, whereas Brucella melitensis strain 16M expresses longer polymer consisting mostly of M-type epitopes. Proposed explanation was that epitope A is defined by 1-2-linked homopolymer of N-formylperosamine (Rha4NFo), while epitope M is a pentasaccharide with four 2- and one 3-substituted Rha4NFo. We reinvestigated both types of structures by 2D NMR and showed that M-epitope is a tetrasaccharide, missing one of the 2-linked Rha4NFo as compared to the previously proposed structure. Polysaccharide from B. melitensis 16M contains a fragment of 1-2-linked polymer, capped with M-type polymer. Other strains contain one or two M-type units at the non-reducing end of the 1-2-linked O-chain.
NMR, LPS, structure, O-antigens, O-specific, O-specific polysaccharide, Brucella, MS, Brucella melitensis
Structure type: homopolymer
Location inside paper: p.145
Trivial name: perosamine homopolymer, perosamine
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_131172,IEDB_134281,IEDB_1397515,IEDB_2116320,IEDB_434547,IEDB_628715
Methods: 13C NMR, 1H NMR, NMR-2D, GC-MS, sugar analysis, MALDI-TOF MS, NMR-1D, N-acetylation, de-N-acelation
Comments, role: Part of the native polysaccharide structure (see RR: 29626). B. abortus and B. suis polysaccharides contained up to 16 RhaNFo residues,and B. melitensis strains 3 and 16 M,contained up to 34 RhaNFo residues.
Related record ID(s): 29248, 29626, 29627, 29628, 29630, 29631
NCBI Taxonomy refs (TaxIDs): 29459,
235,
29461Reference(s) to other database(s): GTC:G55700WU, GlycomeDB:
3393, CCSD:
42651, CBank-STR:4424
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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