Lin FL, Vinogradov E, Deng C, Zeller S, Green BA, Jansen KU, Pavliak V Identification of the common antigenic determinant shared by Streptococcus pneumoniae serotypes 33A, 35A, and 20 capsular polysaccharides Carbohydrate Research380 (2013)
101-107
NCBI PubMed ID:23981856 Publication DOI:10.1016/j.carres.2013.08.001 Journal NLM ID:0043535 Publisher: Elsevier Correspondence: fiona.linpfizer.com Institutions: Pfizer Vaccine Research, 401 N. Middletown Road, Pearl River, NY 10965, USA
In order to better understand cross-reactions of serogroup 33 polysaccharides and the typing sera, the structure of pneumococcal capsular polysaccharide serotype 33A was elucidated. Serotype 33A has been shown to have an identical polysaccharide backbone as that of serotype 33F, with two additional sites of O-acetylation at C5, and C6 of the 3-β-Galf residue in serotype 33A. This finding is consistent with the presence of an additional functional acetyltransferase gene (wcjE) in the cps biosynthetic locus of serotype 33A compared to 33F. The identical polysaccharide backbone with at least one common O-acetylation site (C2 of 5-β-Galf) shared by serotype 33A and 33F polysaccharides is proposed to be the epitope recognized by typing serum 33b. In addition, a 5,6-di-O-acetylated →3)-β-D-Galf5,6Ac-(1→3)-β-D-Glcp-(1→ disaccharide unit, a common structural motif present in serotypes 33A, 20, and 35A polysaccharides, is proposed to be the antigenic determinant recognized by typing serum 20b.
Methods: 13C NMR, 1H NMR, NMR-2D, GC-MS, sugar analysis, acid hydrolysis, MS/MS, de-O-acetylation, NMR-1D, HPAEC-PAD, CE-MS Comments, role: published polymerization frame was shifted for conformity with other records
Related record ID(s): 29259, 29635, 29637 NCBI Taxonomy refs (TaxIDs):1313 Reference(s) to other database(s): GTC:G10341HC Show glycosyltransferases
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