Taxonomic group: bacteria / Bacteroidetes
(Phylum: Bacteroidetes)
Host organism: Homo sapiens
Associated disease: periodontitis [ICD11:
DA0C 
]
The structure was elucidated in this paperNCBI PubMed ID: 23616409Publication DOI: 10.1128/CVI.00139-13Journal NLM ID: 101252125Publisher: American Society for Microbiology
Correspondence: christina.schaeffer
boku.ac.at; Otto Holst <oholst
fz-borstel.de>
Institutions: Department of NanoBiotechnology, NanoGlycobiology Unit, Universitat fur Bodenkultur Wien, Vienna, Austria
Tannerella forsythia is a Gram-negative anaerobic organism that inhabits subgingival plaque biofilms and is covered with a so far unique surface layer composed of two glycoproteins. It belongs to the so-called 'red complex' of bacteria comprising species that are associated with periodontal disease. While the surface layer glycoprotein glycan structure had been elucidated recently and found to be a virulence factor, no structural data on the lipopolysaccharide (LPS) of this organism were available. In this study, the T. forsythia LPS structure was partially elucidated by a combined mass spectrometry (MS) and nuclear magnetic resonance spectroscopy (NMR) approach and initial experiments to characterize its immunostimulatory potential were performed. The T. forsythia LPS is a complex, rough-type LPS with a core region composed of one 3-deoxy-d-manno-oct-2-ulosonic acid (Kdo) residue, three mannose residues, and two glucosamine residues. MS analyses of O-deacylated LPS proved that, in addition, one phosphoethanolamine residue and most likely one galactose-phosphate residue were present, however, their positions could not be identified. Stimulation of human macrophages with T. forsythia LPS resulted in the production of the proinflammatory cytokines interleukin-1 (IL-1), IL-6, and tumor necrosis factor alpha in a dose-dependent manner. The response to T. forsythia LPS was observed only upon stimulation in the presence of fetal calf serum (FCS), whereas no cytokine production was observed in the absence of FCS. This finding suggests that the presence of certain additional cofactors is crucial for the immune response induced by T. forsythia LPS.
Lipopolysaccharide, structure, Kdo, core region, Biofilm, cytokines, Tannerella forsythia
Structure type: oligomer
Location inside paper: p.950, fig.4
Compound class: LPS
Contained glycoepitopes: IEDB_130650,IEDB_130701,IEDB_135394,IEDB_141793,IEDB_141807,IEDB_144983,IEDB_151531,IEDB_152206,IEDB_153220,IEDB_164174,IEDB_983930,SB_197,SB_198,SB_44,SB_67,SB_72,SB_73
Methods: 13C NMR, 1H NMR, NMR-2D, PCR, SDS-PAGE, acid hydrolysis, ESI-ICR-MS, de-O-acylation with hydrazine, composition analysis, NMR-1D, de-N-acylation, statistical analysis
Biological activity: cytokine production of U-937 macrophages upon LPS stimulation
Comments, role: de-O-acylated R-type LPS
Related record ID(s): 29322
NCBI Taxonomy refs (TaxIDs): 203275
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There is only one chemically distinct structure:
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