Potekhina NV, Shashkov AS, Streshinskaia GM, Tul'skaya EM, Senchenkova SN, Kudryashova EB, Dmitrenok AS Disaccharide 1-phosphate polymers of some representatives of the Bacillus subtilis group Biochemistry (Moscow)78(10) (2013)
1146-1154
The structure was elucidated in this paper NCBI PubMed ID:24237149 Publication DOI:10.1134/S000629791310009X Journal NLM ID:0376536 Publisher: Nauka/Interperiodica Correspondence: potekhina56mail.ru Institutions: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Biological Faculty, Lomonosov Moscow State University, Moscow, Russia, All-Russian Collection of Microorganisms, Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr. Nauki 5, 142290, Pushchino, Moscow Region, Russia, fax: (495) 9394309
Disaccharide 1-phosphate polymers as well as teichoic acids of various structures have been found in the cell walls of the representatives of the Bacillus subtilis group, namely Bacillus subtilis subsp. spizizenii VKM B-720 and VKM B-916, B. subtilis VKM B-517, and Bacillus vallismortis VKM B-2653(T). Disaccharide 1-phosphate polymers are composed of repeating units of the following structure: -P-4)-β-D-GlcpNAc-(1→6)-α-D-Galp-(1-, the N-acetylglucosamine residues are partially acetylated at positions O3 and O6 (VKM B-720 and VKM B-916); -P-4)-β-D-Glcp-(1→6)-α-D-GlcpNAc-(1-, the glucopyranose residues are partially acetylated at positions O2 or O3 (VKM B-517); -P-6)-α-D-GlcpNH3(+)/α-D-GlcpNAc-(1→2)-α-D-Glcp-(1-, the N-acetylglucosamine residues are partially deacetylated (VKM B-2653(T)). The structures of the two last disaccharide 1-phosphate polymers have not been reported so far for Gram-positive bacteria. The teichoic acids in the studied strains are O-D-alanyl-1,5-poly(ribitol phosphates) substituted with β-D-glucopyranose (VKM B-517, VKM B-720, VKM B-916) or 2-acetamido-2-deoxy-β-D-glucopyranose (VKM B-2653(T)). The structures of the phosphate-containing polymers have been studied by chemical methods and by NMR spectroscopy.
Methods: 13C NMR, 1H NMR, NMR-2D, HF solvolysis, sugar analysis, 31P NMR, acid hydrolysis, paper chromatography, NMR-1D, paper electrophoresis Comments, role: main fragments of the major polymer with irregular distribution of the Glcp residues in the chain.
Related record ID(s): 29323, 29729, 29730, 29731, 29732, 29733, 29734, 29735, 29736, 29737, 29738, 29740, 29741, 29742, 29743 NCBI Taxonomy refs (TaxIDs):1423 Reference(s) to other database(s): GTC:G03310MH Show glycosyltransferases
Found 
report error
mail.ru