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Taxonomic group: bacteria / Firmicutes (Phylum: Firmicutes)
Associated disease: infection due to Streptococcus pneumoniae [ICD11: XN3PW

]

NCBI PubMed ID: 9562629
Journal NLM ID: 0376600
Publisher: Japanese Biochemical Society
Correspondence: zeijst

vermic.dgk.ruu.nl
Institutions: Department of Bacteriology, Institute of Infectious Diseases and Immunology, School of Veterinary Medicine, Universiteit Utrecht, P.O. Box 80.165, 3508 TD Utrecht, The Netherlands, Institute of Infectious Diseases and Immunology, School of Veterinary Medicine, University of Utrecht, Utrecht, The Netherlands

Streptococcus pneumoniae comprises 90 serotypes, each one having its own specific polysaccharide capsule. In order to explore the diversity of capsular polysaccharide synthesis (cps) gene clusters in S. pneumoniae, we performed cross-hybridizations between the 12 cps genes of S. pneumoniae serotype 14 and chromosomal DNA of 26 strains comprising 26 different capsule types. Large variations in the hybridization patterns were observed. The genes cps14A to cps14D are conserved in most serotypes. Sequences homologous to cps14I to cps14L were only observed in the four types of serogroup 15, which all have a capsule structure similar to that of type 14. By using a cps14E knock-out construct, cpsE mutants of the pneumococcal types 9N, 13, and 15B were obtained. These mutants were unencapsulated and showed reduced glycosyltransferase activity, indicating that the pneumococcal types 9N, 13, and 15B express a glucosyl-1-phosphate transferase which is homologous to Cps14E. Glycosyltransferase assays showed that among 21 pneumococcal types which contain glucose in the core of their capsule polysaccharide, 19 types express glucosyl-1-phosphate transferase activity. However, not all of these types hybridized strongly with Cps14E, the type 14 glucosyl-1-phosphate transferase gene. Thus, pneumococci possess glucosyltransferase genes distinct from cps14E, but encoding enzymes with identical activity. All serotypes which synthesized lipid-linked lactose intermediates in glycosyltransferase activity assays (type 11B, 13, 15F, 15A, 15B, 15C) hybridized with cps14G. This gene encodes a galactosyltransferase which catalyzes the addition of 1,4-linked β-galactose to lipid-linked glucose. The cps14G homologues in type 11B, 13, 15F, 15A, 15B, and 15C may encode a similar β-galactosyltransferase activity as cps14G in type 14.

synthesis, gene, capsular, polysaccharide, Streptococcus, Streptococcus pneumoniae, capsular polysaccharide, cluster, gene cluster, glycosyltransferases, polysaccharides, capsule, diversity

Structure type: polymer chemical repeating unit
Location inside paper: Fig.2, Type 15B, 15C
Compound class: CPS
Contained glycoepitopes: IEDB_115009,IEDB_116046,IEDB_130646,IEDB_130697,IEDB_131186,IEDB_135813,IEDB_135818,IEDB_136044,IEDB_136906,IEDB_137340,IEDB_137472,IEDB_137776,IEDB_1391966,IEDB_1392542,IEDB_140108,IEDB_140110,IEDB_140122,IEDB_140624,IEDB_141794,IEDB_141807,IEDB_142351,IEDB_142487,IEDB_142488,IEDB_143634,IEDB_146664,IEDB_149138,IEDB_149139,IEDB_149141,IEDB_149142,IEDB_149143,IEDB_149144,IEDB_149145,IEDB_149147,IEDB_149148,IEDB_149149,IEDB_149150,IEDB_149151,IEDB_151528,IEDB_151531,IEDB_167072,IEDB_190606,IEDB_742245,IEDB_983931,SB_145,SB_165,SB_166,SB_173,SB_187,SB_192,SB_195,SB_30,SB_6,SB_7,SB_88

Methods: 1H NMR, IR, FAB-MS
Biosynthesis and genetic data: genetic data
Comments, role: in S. pneumonia 15B degree of O-acetylation at unknown position is ca. 70%; structure of 15B revised in ID 10063

Related record ID(s): 2849, 2972, 2973, 133128
NCBI Taxonomy refs (TaxIDs): 1313
Show glycosyltransferases

There is only one chemically distinct structure:

*OC[C@H]1O[C@@H](O[C@H]2[C@@H](O)[C@@H](CO)O[C@@H](O[C@@H]3[C@@H](CO)O[C@@H](*)[C@H](O)[C@H]3O)[C@@H]2O)[C@H](NC(C)=O)[C@@H](O)[C@@H]1O[C@@H]1O[C@H](CO)[C@H](O)[C@H](OP(=O)(O)O)[C@H]1O[C@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O

SVG MW SMILES 3D mol Ionize