Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: pneumonia [ICD11:
CA40 
];
bacteremia [ICD11:
MA15.0 ];
infection due to Pseudomonas aeruginosa [ICD11:
XN5L6 ]
The structure was elucidated in this paperNCBI PubMed ID: 23664878Publication DOI: 10.1016/j.bmcl.2013.04.051Journal NLM ID: 9107377Publisher: Elsevier
Correspondence: I. Brockhausen <brockhau
queensu.ca>
Institutions: Department of Medicine, Queen's University, Kingston, Ontario, Canada K7L 3N6, Department of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario, Canada K7L 3N6, Carlsberg Laboratory, Copenhagen-V DK-1799, Denmark
Gram negative bacteria have lipopolysaccharides (LPS) that are critical for their survival. LPS molecules are composed of antigenic exopolysaccharide chains (O antigens). We are interested in discovering the enzymes involved in the biosynthesis of O antigens in Pseudomonas aeruginosa. The common polysaccharide antigen contains α-linked d-rhamnose residues. We have now synthesized GDP-d-rhamnose by a convenient synthesis in aqueous solution, and have shown that it can be used without extensive purification as the donor substrate for d-rhamnosyltransferase (WbpZ) from the P. aeruginosa strain PAO1. The availability of this nucleotide sugar preparation allows for characterization of d-rhamnosyltransferases.
Pseudomonas aeruginosa, O-antigens, D-Rha-transferase, WbpZ, GDP-D-Rha
Structure type: oligomer
Location inside paper: p.3492, fig.1
Aglycon: phenylundecanol (PhU)
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_137340,IEDB_1394181,IEDB_141807,IEDB_150077,IEDB_151531
Methods: 1H NMR, glycosyltransferase assays, ESI-MS, MALDI-MS, Western blotting, chemical synthesis, biochemical methods
Enzymes that release or process the structure: WbpZ, D-rhamnosyltransferase
Synthetic data: chemoenzymatic
Related record ID(s): 29385
NCBI Taxonomy refs (TaxIDs): 208964
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
report error