Taxonomic group: bacteria / Chlamydiae
(Phylum: Chlamydiae)
Associated disease: infection due to Chlamydia [ICD11:
XN27H 
]
NCBI PubMed ID: 23295476Publication DOI: 10.1107/S1744309112047422Journal NLM ID: 101226117Publisher: Oxford, England: Wiley-Blackwell
Correspondence: cbrooks1
ualberta.ca; svevans
uvic.ca
Institutions: Department of Biochemistry and Microbiology, University of Victoria, PO Box 3055 STN CSC, Victoria, BC V8W 3P6, Canada
The near-germline antibody S25-2 exhibits a remarkable cross-reactivity for oligosaccharides containing the bacterial lipopolysaccharide carbohydrate 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo). The recent synthesis of a variety of Kdo analogues permits a detailed structural analysis of the importance of specific interactions in antigen recognition by S25-2. The Kdo disaccharide analogue Kdo-(2→4)-5,6-dehydro-Kdo lacks a 5-OH group on the second Kdo residue and has been cocrystallized with S25-2. The structure reveals that the modification of the Kdo residue at position 5 results in a rearrangement of intramolecular hydrogen bonds in the antigen that allows it to assume a novel conformation in the antibody-combining site. The cross-reactive binding of S25-2 to this synthetic ligand highlights the adaptability of this antibody to non-natural synthetic analogues.
5, 6-dehydro-Kdo disaccharide, 4hgw, antibody S25-2
Structure type: oligomer
Location inside paper: p.3, fig.1 (b)
Trivial name: core-lipid A carbohydrate backbone, epitope G
Compound class: core oligosaccharide, LPS
Contained glycoepitopes: IEDB_130650,IEDB_130657,IEDB_130658,IEDB_130659,IEDB_135394,IEDB_141807,IEDB_150760,IEDB_150908,IEDB_151531
Methods: crystallization
3D data: 3D data
NCBI Taxonomy refs (TaxIDs): 810Reference(s) to other database(s): GlycomeDB:
8180, CCSD:
46734, CBank-STR:16077
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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