Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Brucella melitensis [ICD11:
XN7ZW 
]
NCBI PubMed ID: 23567915Publication DOI: 10.1016/j.bbamem.2013.03.029Journal NLM ID: 0217513Publisher: Elsevier
Correspondence: B.B. Bonev <Boyan.Bonev
nottingham.ac.uk>
Institutions: School of Biomedical Sciences, University of Nottingham, Nottingham NG7 2UH, UK, Departamento de Microbiología, Universidad de Navarra, e Instituto de Salud Tropical, 31008 Pamplona, Spain
Lipopolysaccharide (LPS) is a major component of the external leaflet of bacterial outer membranes, key pro-inflammatory factor and an important mediator of host-pathogen interactions. In host cells it activates the complement along with a pro-inflammatory response via a TLR4-mediated signalling cascade and shows preference for cholesterol-containing membranes. Here, we use solid state (13)C and (31)P MAS NMR to investigate the interactions of LPS from three bacterial species, Brucella melitensis, Klebsiella pneumoniae and Escherichia coli, with mixed lipid membranes, raft models. All endotoxin types are found to be pyrophosphorylated and Klebsiellar LPS is phosphonylated, as well. Carbon-13 MAS NMR indicates an increase in lipid order in the presence of LPS. Longitudinal (31)P relaxation, providing a direct probe of LPS molecular and segmental mobility, reveals a significant reduction in (31)P T1 times and lower molecular mobility in the presence of ternary lipid mixtures. Along with the ordering effect on membrane lipid, this suggests a preferential partitioning of LPS into ordered bilayer sphingomyelin/cholesterol-rich domains. We hypothesise that this is an important evolutionary drive for the selection of GPI-anchored raft-associated LPS-binding proteins as a first line of response to membrane-associated LPS.
LPS, endotoxin, host-pathogen interactions, lipid domains, high resolution solid state NMR, longitudinal relaxation
Structure type: polymer chemical repeating unit
Location inside paper: p.1732, fig.1D
Trivial name: M antigen
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130647,IEDB_131172,IEDB_134279,IEDB_134280,IEDB_134281,IEDB_1397515,IEDB_2116320,IEDB_434544,IEDB_434545,IEDB_434546
Methods: NMR
Biological activity: receptor-independent interactions between LPS with model membranes and lipid membranes
Comments, role: published polymerization frame was shifted for conformity with other records; published polymerization frame was shifted for conformity with other records.
Related record ID(s): 29457, 29458
NCBI Taxonomy refs (TaxIDs): 29459Reference(s) to other database(s): GTC:G53253RW, GlycomeDB:
3021
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There is only one chemically distinct structure:
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