Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: neonatal meningitis [ICD11:
KA65.4 
, Life stage: neonatal];
neonatal necrotising enterocolitis [ICD11:
KB88 , Life stage: neonatal];
septicemia [ICD11:
MA15.Y ]
The structure was elucidated in this paperNCBI PubMed ID: 23584238Publication DOI: 10.1016/j.carres.2013.03.003Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: margo
chem.univ.gda.pl
Institutions: Faculty of Chemistry, University of Gdansk, Gdansk, Poland, School of Science and Technology, Nottingham Trent University, Clifton Lane, Nottingham NG11 8NS, United Kingdom, Department of Molecular Microbiology and Serology, National Salmonella Centre, Medical University of Gdansk, Do Studzienki 38, 80-227 Gdansk, Poland
The Cronobacter spp. are Gram-negative bacterial pathogens that can cause infections in all age groups, and have a high mortality rate in neonates due to necrotizing enterocolitis and meningitis. Recent genotyping studies have revealed a strong clonal lineage in the genus, but this has not been compared with physiological traits. The O-polysaccharides (OPS) were isolated from three C. turicensis sequence type 5 strains (57, 564, and 566) and structurally characterized using (1)H and (13)C NMR spectroscopy, including two-dimensional DQF-COSY, TOCSY, ROESY, and HSQC analysis. Further compositional determination was undertaken using classical chemical methods followed by GLC, and GLC-MS analysis. The repeating unit of the isolated O-polysaccharides consists of GlcNAc, Rha, Glc, and had the structure shown below and therefore complemented the sequence type.
NMR, structure, O-polysaccharide, Cronobacter sakazakii, Cronobacter turicensis
Structure type: polymer chemical repeating unit
Location inside paper: abtract, p.90
Compound class: O-polysaccharide
Contained glycoepitopes: IEDB_135813,IEDB_137340,IEDB_141807,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_151531,IEDB_225177,IEDB_885823,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, GLC-MS, NMR-2D, GLC, chemical methods, composition analysis
NCBI Taxonomy refs (TaxIDs): 413502,
1208590Reference(s) to other database(s): GTC:G31196CL
Show glycosyltransferases
NMR conditions: in D2O at 329 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
4,3,2 Ac 174.95 23.55
4,3 bDGlcpN 104.09 56.01 82.69 70.78 76.34 62.11
4,2 aDGlcp 100.13 73.12 74.15 70.12 72.44 61.40
4 bLRhap 102.75 77.02 81.11 73.12 73.78 17.57
2 Ac 175.51 23.47
bDGlcpN 102.02 57.07 74.21 78.79 75.72 61.91
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
4,3,2 Ac - 2.102
4,3 bDGlcpN 4.740 3.735 3.852 3.522 3.480 3.807-3.986
4,2 aDGlcp 5.297 3.511 3.797 3.523 4.314 3.924-3.956
4 bLRhap 5.008 4.408 3.764 3.509 3.444 1.387
2 Ac - 2.080
bDGlcpN 4.643 3.725 3.805 3.691 3.577 3.854-4.004
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
4,3,2 Ac 23.55/2.102
4,3 bDGlcpN 104.09/4.740 56.01/3.735 82.69/3.852 70.78/3.522 76.34/3.480 62.11/3.807-3.986
4,2 aDGlcp 100.13/5.297 73.12/3.511 74.15/3.797 70.12/3.523 72.44/4.314 61.40/3.924-3.956
4 bLRhap 102.75/5.008 77.02/4.408 81.11/3.764 73.12/3.509 73.78/3.444 17.57/1.387
2 Ac 23.47/2.080
bDGlcpN 102.02/4.643 57.07/3.725 74.21/3.805 78.79/3.691 75.72/3.577 61.91/3.854-4.004
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 4,3,2 | Ac |
| 2.102 | |
| 4,3 | bDGlcpN | 4.740 | 3.735 | 3.852 | 3.522 | 3.480 | 3.807
3.986 |
| 4,2 | aDGlcp | 5.297 | 3.511 | 3.797 | 3.523 | 4.314 | 3.924
3.956 |
| 4 | bLRhap | 5.008 | 4.408 | 3.764 | 3.509 | 3.444 | 1.387 |
| 2 | Ac |
| 2.080 | |
| | bDGlcpN | 4.643 | 3.725 | 3.805 | 3.691 | 3.577 | 3.854
4.004 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 4,3,2 | Ac | 174.95 | 23.55 | |
| 4,3 | bDGlcpN | 104.09 | 56.01 | 82.69 | 70.78 | 76.34 | 62.11 |
| 4,2 | aDGlcp | 100.13 | 73.12 | 74.15 | 70.12 | 72.44 | 61.40 |
| 4 | bLRhap | 102.75 | 77.02 | 81.11 | 73.12 | 73.78 | 17.57 |
| 2 | Ac | 175.51 | 23.47 | |
| | bDGlcpN | 102.02 | 57.07 | 74.21 | 78.79 | 75.72 | 61.91 |
|
There is only one chemically distinct structure:
Found 
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