Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: cystic fibrosis (CF) [ICD11:
CA25 
];
infection due to Pseudomonas aeruginosa [ICD11:
XN5L6 ]
NCBI PubMed ID: 23354750Publication DOI: 10.1128/JB.01999-12Journal NLM ID: 2985120RPublisher: American Society for Microbiology
Correspondence: joanna.goldberg
emory.edu
Institutions: Department of Microbiology, Immunology, and Cancer Biology, University of Virginia Health Sciences Center, Charlottesville, VA, USA
Pseudomonas aeruginosa in the lungs of cystic fibrosis patients is characterized by a series of genotypic and phenotypic changes that reflect the transition from acute to chronic infection. These include the overproduction of the exopolysaccharide alginate and the loss of complete lipopolysaccharide (LPS). LPS is a major component of the Gram-negative outer-membrane and is composed of lipid A, core oligosaccharide, and O-antigen. In this report, we show that the LPS-defect of the P. aeruginosa chronic infection isolate strain 2192 is temperature sensitive. When grown at 25 degrees C, 2192 expresses serotype O1 LPS of moderate chain length and reduced amount when compared to a wild-type serotype O1 laboratory strain (stO1). In contrast, 2192 does not express any LPS O antigen when grown at 37 degrees C. This is the first time that a temperature-sensitive defect in O antigen production has been reported. Using complementation analyses with a constructed wbpM deletion mutant of stO1, we demonstrate that the temperature-sensitive O antigen production defect in 2192 is due to a mutation in wbpM, which encodes a UDP-4,6-GlcNAc dehydratase involved in O-antigen synthesis. The mutation, a deletion of a single amino acid (V636) from the extreme C-terminus of WbpM, renders the protein less stable than its wild-type counterpart. This residue of WbpM, which is critical for stability and function, is located outside of the recognized domains of the protein and may provide more insight into the structure-function relationship of this enzyme, which is found in all 20 serotypes of P. aeruginosa. We also identify a promoter of wbpM and map a transcriptional start site of wbpM and show that mucoidy plays a role in the loss of expression of high molecular weight LPS in this CF isolate.
Pseudomonas aeruginosa, lipopolysaccharide O-antigen, cystic fibrosis
Structure type: polymer chemical repeating unit
Location inside paper: p.1505
Trivial name: B-band polysaccharid
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130648,IEDB_137473,IEDB_1391961,IEDB_141584,IEDB_885822
Methods: genetic methods
Biosynthesis and genetic data: genetic data
NCBI Taxonomy refs (TaxIDs): 287Reference(s) to other database(s): GTC:G15870HS, GlycomeDB:
3409
Show glycosyltransferases
There is only one chemically distinct structure:
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