Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: nontyphoidal Salmonella (NTS) disease [ICD11:
1A09 
, ICD11:
XN0QE ];
NTS meningitis [ICD11:
1A09 , ICD11:
XN0QE ];
NTS bacteremia [ICD11:
XN0QE ];
infection due to Salmonella enterica [ICD11:
XN5VC ]
NCBI PubMed ID: 24384528Publication DOI: 10.1016/j.carres.2013.12.003Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: francesca.micoli
novartis.com
Institutions: Department of Chemistry, University of Cape Town, Rondebosch 7701, South Africa, Novartis Vaccines Institute for Global Health, Via Fiorentina 1, I-53100 Siena, Italy, Dipartimento di Scienze della Vita, Ed. C11, Università di Trieste, via L. Giorgieri 1, 34127 Trieste, Italy, Medical Research Council Centre for Immune Regulation, Institute of Biomedical Research, School of Immunity and Infection, College of Medicine and Dental Sciences, University of Birmingham, Birmingham B15 2TT, UK
Salmonella Typhimurium is the major cause of invasive nontyphoidal Salmonella disease in Africa, with high mortality among children and HIV-infected individuals. Currently, no vaccine is available for use in humans. Antibodies directed against the O-polysaccharide of the lipopolysaccharide molecule of Salmonella mediate bacterial killing and are protective, and conjugation of the O-polysaccharide to a carrier protein represents a possible strategy for vaccine development. Here we have purified the O-polysaccharide from six different strains of S. Typhimurium and fully characterized them using analytical methods including HPLC-SEC, HPAEC-PAD, GC, GC-MS, 1D and 2D NMR spectroscopy. All the O-polysaccharide samples showed a similar bimodal molecular mass distribution, but differed with respect to the amount and position of O-acetylation and glucosylation. For some strains, O-acetyl groups were found not only on C-2 of abequose (factor 5 specificity), but also on C-2 and C-3 of rhamnose; glucose was found to be linked 1→4 or 1→6 to galactose in different amounts according to the strain of origin. This structural variability could have an impact on the immunogenicity of corresponding glycoconjugate vaccines and different strains need to be evaluated in order to identify the appropriate source of O-polysaccharide to use for the development of a candidate conjugate vaccine with broad coverage against S. Typhimurium.
O-polysaccharide, bacterial polysaccharide structure, Salmonella typhimurium
Structure type: polymer chemical repeating unit
Location inside paper: p.2, fig.1
Compound class: O-polysaccharide
Contained glycoepitopes: IEDB_127517,IEDB_130422,IEDB_130701,IEDB_135509,IEDB_135513,IEDB_135514,IEDB_135611,IEDB_136093,IEDB_136105,IEDB_136775,IEDB_136906,IEDB_137472,IEDB_137486,IEDB_141794,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_152206,IEDB_153307,IEDB_190606,IEDB_225177,IEDB_840978,IEDB_885823,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_67,SB_7,SB_72
Methods: 13C NMR, 1H NMR, methylation, GLC-MS, NMR-2D, sugar analysis, acid hydrolysis, GLC, mild acid hydrolysis, de-O-acetylation, NMR-1D, HPLC, HPAEC-PAD, SEC
Related record ID(s): 30414, 30415, 30416
NCBI Taxonomy refs (TaxIDs): 90371
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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(later renamed to: Salmonella enterica ssp. enterica sv. Typhimurium)