Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Enterobacter cloacae [ICD11:
XN3YM 
]
The structure was elucidated in this paperNCBI PubMed ID: 24530689Publication DOI: 10.1016/j.carres.2014.01.001Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: A.V. Perepelov <perepel
ioc.ac.ru>
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China, Tianjin Key Laboratory for Microbial Functional Genomics, TEDA College, Nankai University, TEDA, Tianjin, China
The O-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of Enterobacter cloacae G2277 and studied by sugar analysis along with 1D and 2D (1)H and (13)C NMR spectroscopy. The following structure of the linear pentasaccharide repeating unit was established, where a galacturonic acid (GalA) residue is mono-O-acetylated at position either 2 or 3: The O-antigen gene cluster of E. cloacae G2277 was sequenced. The gene functions were tentatively assigned by comparison with sequences in the available databases and found to be in agreement with the O-polysaccharide structure.
Lipopolysaccharide, O-polysaccharide, bacterial polysaccharide structure, O-antigen gene cluster, Enterobacter cloacae
Structure type: polymer chemical repeating unit
Location inside paper: abstract, p.11
Compound class: O-polysaccharide
Contained glycoepitopes: IEDB_133754,IEDB_136105,IEDB_137340,IEDB_141807,IEDB_144825,IEDB_151531,IEDB_225177,IEDB_885823
Methods: 13C NMR, 1H NMR, NMR-2D, DNA sequencing, sugar analysis, acid hydrolysis, GLC, mild acid hydrolysis, de-O-acetylation, NMR-1D, function analysis of gene clusters
Related record ID(s): 30423
NCBI Taxonomy refs (TaxIDs): 550
Show glycosyltransferases
There is only one chemically distinct structure:
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