Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: bacillary dysentery (shigellosis) [ICD11:
1A02 
, ICD11:
SA56 , ICD11:
XN7HG ];
infection due to Shigella flexneri [ICD11:
XN7Y2 ]
The structure was elucidated in this paperNCBI PubMed ID: 25327486Publication DOI: 10.1186/s12866-014-0266-7Journal NLM ID: 100966981Publisher: Biomed Central
Correspondence: yknirel
gmail.com
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, China CDC, P.O. Box 5, Changping, Beijing, China, School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney NSW 2052, Australia
O-antigen (O-polysaccharide) of the lipopolysaccharide is a highly variable cell component of the outer membrane in Shigella flexneri. It defines the serospecificity and plays an important role in the pathogenesis of shigellosis. There are two distinct O-antigen forms for the 19 serotypes of S. flexneri: one for serotypes 1-5, X, Y, 7 (and their subtypes), and the other for serotype 6. Although having different basal O-polysaccharide structures, the two forms share a common disaccharide fragment [→2)-α-l-Rhap III-(1 → 2)-α-l-Rhap II]. In serotype 6 and some non-6 serotypes, RhaIII is O-acetylated at position either 3 or 4 (3/4-O-acetylation), conferring to the hosts a novel antigenic determinant named O-factor 9. An acyltransferase gene (oacB) responsible for this modification has been identified in serotypes 1a, 1b, 2a, 5a, and Y, but not in serotype 6.
O-antigen, Shigella flexneri, 3/4-O-acetylation, aAcyltransferase, oacC, anti-O-factor 9 serum
Structure type: polymer chemical repeating unit
Location inside paper: p.266-7, fig.3, Sf301, Sf301∆oacB_pSQZ4, Sf301∆oacB_pSQZ5
Compound class: O-polysaccharide
Contained glycoepitopes: IEDB_125613,IEDB_125614,IEDB_127514,IEDB_130687,IEDB_133752,IEDB_133753,IEDB_133754,IEDB_135806,IEDB_135807,IEDB_135808,IEDB_135809,IEDB_135813,IEDB_135817,IEDB_135849,IEDB_136105,IEDB_137340,IEDB_141807,IEDB_141815,IEDB_141816,IEDB_142488,IEDB_143253,IEDB_144998,IEDB_146664,IEDB_151531,IEDB_153213,IEDB_225177,IEDB_885823,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, PCR, SDS-PAGE, DNA techniques, Western blotting, serological methods, GPC, bioinformatic analysis
Comments, role: The total degree of 3/4-O-acetylation are 90% for S. flexneri Sf301∆oacB_pSQZ4 and 55% for S. flexneri Sf301∆oacB_pSQZ5. Shigella flexneri strain Sf301 belongs to serotype 2a; Shigella flexneri Sf301∆oacB is the strain Sf301 with the oacB gene inactive; Shigella flexneri Sf301∆oacB_pSQZ4 and Sf301∆oacB_pSQZ5 are S. flexneri strains Sf301∆oacB transformed by plasmids pSQZ4 and pSQZ5, respectively.
Related record ID(s): 30083, 30314, 30315, 30316, 30318
NCBI Taxonomy refs (TaxIDs): 42897
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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